2osv
From Proteopedia
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'''Crystal Structure of ZnuA from E. coli''' | '''Crystal Structure of ZnuA from E. coli''' | ||
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[[Category: Jogl, G.]] | [[Category: Jogl, G.]] | ||
[[Category: Li, H.]] | [[Category: Li, H.]] | ||
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Revision as of 08:35, 4 May 2008
Crystal Structure of ZnuA from E. coli
Overview
Bacterial ATP-binding cassette transport systems for high-affinity uptake of zinc and manganese use a cluster 9 solute-binding protein. Structures of four cluster 9 transport proteins have been determined previously. However, the structural determinants for discrimination between zinc and manganese remain under discussion. To further investigate the variability of metal binding sites in bacterial transporters, we have determined the structure of the zinc-bound transport protein ZnuA from Escherichia coli to 1.75 A resolution. The overall structure of ZnuA is similar to other solute-binding transporters. A scaffolding alpha-helix forms the backbone for two structurally related globular domains. The metal-binding site is located at the domain interface. The bound zinc ion is coordinated by three histidine residues (His78, His161 and His225) and one glutamate residue (Glu77). The functional role of Glu77 for metal binding is unexpected, because this residue is not conserved in previously determined structures of zinc and manganese-specific transport proteins. The observed metal coordination by four protein residues differs significantly from the zinc-binding site in the ZnuA transporter from Synechocystis 6803, which binds zinc via three histidine residues. In addition, the E. coli ZnuA structure reveals the presence of a disulfide bond in the C-terminal globular domain that is not present in previously determined cluster 9 transport protein structures.
About this Structure
2OSV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of the zinc-binding transport protein ZnuA from Escherichia coli reveals an unexpected variation in metal coordination., Li H, Jogl G, J Mol Biol. 2007 May 18;368(5):1358-66. Epub 2007 Mar 15. PMID:17399739 Page seeded by OCA on Sun May 4 11:35:31 2008
