2oug
From Proteopedia
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[[Image:2oug.gif|left|200px]] | [[Image:2oug.gif|left|200px]] | ||
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'''Crystal structure of the RfaH transcription factor at 2.1A resolution''' | '''Crystal structure of the RfaH transcription factor at 2.1A resolution''' | ||
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[[Category: Vassylyev, D G.]] | [[Category: Vassylyev, D G.]] | ||
[[Category: Vassylyeva, M N.]] | [[Category: Vassylyeva, M N.]] | ||
| - | [[Category: | + | [[Category: Transcription elongation]] |
| - | [[Category: | + | [[Category: Transcription factor]] |
| - | [[Category: | + | [[Category: Transcription pausing]] |
| - | [[Category: | + | [[Category: Virulence]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:40:42 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:40, 4 May 2008
Crystal structure of the RfaH transcription factor at 2.1A resolution
Overview
RfaH, a paralog of the general transcription factor NusG, is recruited to elongating RNA polymerase at specific regulatory sites. The X-ray structure of Escherichia coli RfaH reported here reveals two domains. The N-terminal domain displays high similarity to that of NusG. In contrast, the alpha-helical coiled-coil C domain, while retaining sequence similarity, is strikingly different from the beta barrel of NusG. To our knowledge, such an all-beta to all-alpha transition of the entire domain is the most extreme example of protein fold evolution known to date. Both N domains possess a vast hydrophobic cavity that is buried by the C domain in RfaH but is exposed in NusG. We propose that this cavity constitutes the RNA polymerase-binding site, which becomes unmasked in RfaH only upon sequence-specific binding to the nontemplate DNA strand that triggers domain dissociation. Finally, we argue that RfaH binds to the beta' subunit coiled coil, the major target site for the initiation sigma factors.
About this Structure
2OUG is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural basis for converting a general transcription factor into an operon-specific virulence regulator., Belogurov GA, Vassylyeva MN, Svetlov V, Klyuyev S, Grishin NV, Vassylyev DG, Artsimovitch I, Mol Cell. 2007 Apr 13;26(1):117-29. PMID:17434131 Page seeded by OCA on Sun May 4 11:40:42 2008
