2ouh

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[[Image:2ouh.jpg|left|200px]]
[[Image:2ouh.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2ouh |SIZE=350|CAPTION= <scene name='initialview01'>2ouh</scene>, resolution 2.4&Aring;
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The line below this paragraph, containing "STRUCTURE_2ouh", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+248'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+A+249'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+A+250'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+B+248'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+B+249'>AC5</scene> and <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+B+250'>AC6</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= THBS1, TSP, TSP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_2ouh| PDB=2ouh | SCENE= }}
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|RELATEDENTRY=[[1z78|1Z78]], [[1za4|1ZA4]], [[2ouj|2OUJ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ouh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ouh OCA], [http://www.ebi.ac.uk/pdbsum/2ouh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ouh RCSB]</span>
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}}
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'''Crystal structure of the Thrombospondin-1 N-terminal domain in complex with fractionated Heparin DP10'''
'''Crystal structure of the Thrombospondin-1 N-terminal domain in complex with fractionated Heparin DP10'''
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[[Category: Tan, K.]]
[[Category: Tan, K.]]
[[Category: Wang, J.]]
[[Category: Wang, J.]]
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[[Category: cell adhesion]]
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[[Category: Cell adhesion]]
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[[Category: dp10]]
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[[Category: Dp10]]
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[[Category: fractionated heparin]]
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[[Category: Fractionated heparin]]
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[[Category: hbd]]
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[[Category: Hbd]]
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[[Category: tsp-1]]
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[[Category: Tsp-1]]
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[[Category: tspn-1]]
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[[Category: Tspn-1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:40:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:23:16 2008''
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Revision as of 08:40, 4 May 2008

Image:2ouh.jpg

Template:STRUCTURE 2ouh

Crystal structure of the Thrombospondin-1 N-terminal domain in complex with fractionated Heparin DP10


Overview

Through its interactions with proteins and proteoglycans, thrombospondin-1 (TSP-1) functions at the interface of the cell membrane and the extracellular matrix to regulate matrix structure and cellular phenotype. We have previously determined the structure of the high affinity heparin-binding domain of TSP-1, designated TSPN-1, in association with the synthetic heparin, Arixtra. To establish that the binding of TSPN-1 to Arixtra is representative of the association with naturally occurring heparins, we have determined the structures of TSPN-1 in complex with heparin oligosaccharides containing eight (dp8) and ten (dp10) subunits, by x-ray crystallography. We have found that dp8 and dp10 bind to TSPN-1 in a manner similar to Arixtra and that dp8 and dp10 induce the formation of trans and cis TSPN-1 dimers, respectively. In silico docking calculations partnered with our crystal structures support the importance of arginine residues in positions 29, 42, and 77 in binding sulfate groups of the dp8 and dp10 forms of heparin. The ability of several TSPN-1 domains to bind to glycosaminoglycans simultaneously probably increases the affinity of binding through multivalent interactions. The formation of cis and trans dimers of the TSPN-1 domain with relatively short segments of heparin further enhances the ability of TSP-1 to participate in high affinity binding to glycosaminoglycans. Dimer formation may also involve TSPN-1 domains from two separate TSP-1 molecules. This association would enable glycosaminoglycans to cluster TSP-1.

About this Structure

2OUH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Heparin-induced cis- and trans-Dimerization Modes of the Thrombospondin-1 N-terminal Domain., Tan K, Duquette M, Liu JH, Shanmugasundaram K, Joachimiak A, Gallagher JT, Rigby AC, Wang JH, Lawler J, J Biol Chem. 2008 Feb 15;283(7):3932-41. Epub 2007 Dec 7. PMID:18065761 Page seeded by OCA on Sun May 4 11:40:46 2008

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