2owm

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[[Image:2owm.jpg|left|200px]]
[[Image:2owm.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2owm |SIZE=350|CAPTION= <scene name='initialview01'>2owm</scene>, resolution 3.25&Aring;
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The line below this paragraph, containing "STRUCTURE_2owm", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Residue+A+1001'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Residue+B+1001'>AC2</scene>, <scene name='pdbsite=AC3:Mg+Binding+Site+For+Residue+C+1001'>AC3</scene>, <scene name='pdbsite=AC4:Mg+Binding+Site+For+Residue+D+1001'>AC4</scene>, <scene name='pdbsite=AC5:Adp+Binding+Site+For+Residue+A+1002'>AC5</scene>, <scene name='pdbsite=AC6:Adp+Binding+Site+For+Residue+B+1002'>AC6</scene>, <scene name='pdbsite=AC7:Adp+Binding+Site+For+Residue+C+1002'>AC7</scene> and <scene name='pdbsite=AC8:Adp+Binding+Site+For+Residue+D+1002'>AC8</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Plus-end-directed_kinesin_ATPase Plus-end-directed kinesin ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.4 3.6.4.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= 17E5.250 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5141 Neurospora crassa])
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-->
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|DOMAIN=
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{{STRUCTURE_2owm| PDB=2owm | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2owm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2owm OCA], [http://www.ebi.ac.uk/pdbsum/2owm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2owm RCSB]</span>
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}}
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'''Motor domain of Neurospora crassa kinesin-3 (NcKin3)'''
'''Motor domain of Neurospora crassa kinesin-3 (NcKin3)'''
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[[Category: Muller, J.]]
[[Category: Muller, J.]]
[[Category: Woehlke, G.]]
[[Category: Woehlke, G.]]
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[[Category: adp]]
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[[Category: Adp]]
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[[Category: kinesin]]
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[[Category: Kinesin]]
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[[Category: kinesin-3]]
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[[Category: Kinesin-3]]
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[[Category: motor domain]]
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[[Category: Motor domain]]
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[[Category: motor protein]]
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[[Category: Motor protein]]
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[[Category: neck linker]]
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[[Category: Neck linker]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:49:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:24:15 2008''
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Revision as of 08:49, 4 May 2008

Image:2owm.jpg

Template:STRUCTURE 2owm

Motor domain of Neurospora crassa kinesin-3 (NcKin3)


Overview

Neurospora crassa kinesin NcKin3 belongs to a unique fungal-specific subgroup of small Kinesin-3-related motor proteins. One of its functions appears to be the transport of mitochondria along microtubules. Here, we present the X-ray structure of a C-terminally truncated monomeric construct of NcKin3 comprising the motor domain and the neck linker, and a 3-D image reconstruction of this motor domain bound to microtubules, by cryoelectron microscopy. The protein contains Mg.ADP bound to the active site, yet the structure resembles an ATP-bound state. By comparison with structures of the Kinesin-3 motor Kif1A in different nucleotide states (Kikkawa, M. et al. (2001) Nature (London, U.K.) 411, 439-445), the NcKin3 structure corresponds to the AMPPCP complex of Kif1A rather than the AMPPNP complex. NcKin3-specific differences in the coordination of the nucleotide and asymmetric interactions between adjacent molecules in the crystal are discussed in the context of the unusual kinetics of the dimeric wild-type motor and the monomeric construct used for crystal structure analysis. The NcKin3 motor decorates microtubules at a stoichiometry of one head per alphabeta-tubulin heterodimer, thereby forming an axial periodicity of 8 nm. In spite of unusual extensions at the N-terminus and within flexible loops L2, L8a, and L12 (corresponding to the K-loop of monomeric kinesins), the microtubule binding geometry is similar to that of other members of the kinesin family.

About this Structure

2OWM is a Single protein structure of sequence from Neurospora crassa. Full crystallographic information is available from OCA.

Reference

X-ray Structure and Microtubule Interaction of the Motor Domain of Neurospora crassa NcKin3, a Kinesin with Unusual Processivity(,)., Marx A, Muller J, Mandelkow EM, Woehlke G, Bouchet-Marquis C, Hoenger A, Mandelkow E, Biochemistry. 2008 Feb 19;47(7):1848-1861. Epub 2008 Jan 19. PMID:18205396 Page seeded by OCA on Sun May 4 11:49:06 2008

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