2owq
From Proteopedia
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| - | + | {{STRUCTURE_2owq| PDB=2owq | SCENE= }} | |
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'''Crystal structure of vaccinia virus uracil-DNA glycosylase''' | '''Crystal structure of vaccinia virus uracil-DNA glycosylase''' | ||
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[[Category: Chattopadhyay, D.]] | [[Category: Chattopadhyay, D.]] | ||
[[Category: Schormann, N.]] | [[Category: Schormann, N.]] | ||
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Revision as of 08:49, 4 May 2008
Crystal structure of vaccinia virus uracil-DNA glycosylase
Overview
BACKGROUND: Uracil-DNA glycosylases (UDGs) catalyze excision of uracil from DNA. Vaccinia virus, which is the prototype of poxviruses, encodes a UDG (vvUDG) that is significantly different from the UDGs of other organisms in primary, secondary and tertiary structure and characteristic motifs. It adopted a novel catalysis-independent role in DNA replication that involves interaction with a viral protein, A20, to form the processivity factor. UDG:A20 association is essential for assembling of the processive DNA polymerase complex. The structure of the protein must have provisions for such interactions with A20. This paper provides the first glimpse into the structure of a poxvirus UDG. RESULTS: Results of dynamic light scattering experiments and native size exclusion chromatography showed that vvUDG is a dimer in solution. The dimeric assembly is also maintained in two crystal forms. The core of vvUDG is reasonably well conserved but the structure contains one additional beta-sheet at each terminus. A glycerol molecule is found in the active site of the enzyme in both crystal forms. Interaction of this glycerol molecule with the protein possibly mimics the enzyme-substrate (uracil) interactions. CONCLUSION: The crystal structures reveal several distinctive features of vvUDG. The new structural features may have evolved for adopting novel functions in the replication machinery of poxviruses. The mode of interaction between the subunits in the dimers suggests a possible model for binding to its partner and the nature of the processivity factor in the polymerase complex.
About this Structure
2OWQ is a Single protein structure of sequence from Vaccinia virus. Full crystallographic information is available from OCA.
Reference
Crystal structure of vaccinia virus uracil-DNA glycosylase reveals dimeric assembly., Schormann N, Grigorian A, Samal A, Krishnan R, DeLucas L, Chattopadhyay D, BMC Struct Biol. 2007 Jul 2;7:45. PMID:17605817 Page seeded by OCA on Sun May 4 11:49:27 2008
