1clz
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(New page: 200px<br /> <applet load="1clz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1clz, resolution 2.8Å" /> '''IGG FAB (IGG3, KAPPA...)
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Revision as of 07:20, 18 November 2007
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IGG FAB (IGG3, KAPPA) FRAGMENT (MBR96) COMPLEXED WITH LEWIS Y NONOATE METHYL ESTER
Overview
The crystal structures of the murine BR96 Fab and its human chimera have, been determined in complex with the nonoate methyl ester derivative of, Lewis Y (nLey) at 2.8 A and 2.5 A resolution, respectively. BR96 binds the, carbohydrate in a large pocket which is formed by residues of all CDR, loops except L2. The binding of the carbohydrate is mediated predominantly, by aromatic residues in BR96. Analysis of the structure suggests that BR96, is capable of recognizing a structure larger than the Le(y), tetrasaccharide, providing a possible explanation for its high tumour, selectivity. The structure provides a rationale for mutagenesis, experiments that have resulted in BR96 CDR loop mutants with increased, affinity for nLey and/or tumour cells.
About this Structure
1CLZ is a Protein complex structure of sequences from Mus musculus with NON as ligand. Full crystallographic information is available from OCA.
Reference
The x-ray structure of an anti-tumour antibody in complex with antigen., Jeffrey PD, Bajorath J, Chang CY, Yelton D, Hellstrom I, Hellstrom KE, Sheriff S, Nat Struct Biol. 1995 Jun;2(6):466-71. PMID:7664109
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