1for

From Proteopedia

---

Revision as of 07:23, 18 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1for, resolution 2.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURE DETERMINATION OF AN FAB FRAGMENT THAT NEUTRALIZES HUMAN RHINOVIRUS AND ANALYSIS OF THE FAB-VIRUS COMPLEX

Overview

The crystal structure of Fab17-IA, an antigen-binding fragment from a, murine immunoglobulin that neutralizes human rhinovirus 14 (HRV14), has, been solved to 2.7 A resolution. Fab17-IA crystallized into three, different space groups depending upon the method used to purify the intact, antibody. The structure was determined by use of molecular and isomorphous, replacement methods. The current model has a crystallographic R-factor of, approximately 19% for 10,192 independent reflections between 8 and 2.7 A., Correlation coefficient calculations showed that the Fab17-IA structure, can be fit into the Fab17-IA/HRV14 image reconstruction density to within, 5 A positional accuracy and to within a few degrees of rotation. The, resulting interface of the docked antibody was examined and showed, extensive charge and shape complementarity with the virus surface that was, supported by site-directed mutagenesis experiments. The success of this, approach validates the utility of combining X-ray crystallography with, cryo-electron microscopy of complex macromolecular assemblies.

About this Structure

1FOR is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Structure determination of an Fab fragment that neutralizes human rhinovirus 14 and analysis of the Fab-virus complex., Liu H, Smith TJ, Lee WM, Mosser AG, Rueckert RR, Olson NH, Cheng RH, Baker TS, J Mol Biol. 1994 Jul 8;240(2):127-37. PMID:8027997

Page seeded by OCA on Sun Nov 18 09:30:31 2007