2p6r
From Proteopedia
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'''Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA''' | '''Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6R OCA]. | |
==Reference== | ==Reference== | ||
Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17558417 17558417] | Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17558417 17558417] | ||
| - | [[Category: Archaeoglobus fulgidus]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Buettner, K.]] | [[Category: Buettner, K.]] | ||
[[Category: Hopfner, K P.]] | [[Category: Hopfner, K P.]] | ||
[[Category: Nehring, S.]] | [[Category: Nehring, S.]] | ||
| - | [[Category: | + | [[Category: Archaeal helicase]] |
| - | [[Category: | + | [[Category: Dna repair]] |
| - | [[Category: | + | [[Category: Protein-dna complex]] |
| - | [[Category: | + | [[Category: Sf2 helicase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:29:52 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 09:29, 4 May 2008
Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA
Overview
To reveal the mechanism of processive strand separation by superfamily-2 (SF2) 3'-->5' helicases, we determined apo and DNA-bound crystal structures of archaeal Hel308, a helicase that unwinds lagging strands and is related to human DNA polymerase theta. Our structure captures the duplex-unwinding reaction, shows that initial strand separation does not require ATP and identifies a prominent beta-hairpin loop as the unwinding element. Similar loops in hepatitis C virus NS3 helicase and RNA-decay factors support the idea that this duplex-unwinding mechanism is applicable to a broad subset of SF2 helicases. Comparison with ATP-bound SF2 enzymes suggests that ATP promotes processive unwinding of 1 base pair by ratchet-like transport of the 3' product strand. Our results provide a first structural framework for strand separation by processive SF2 3'-->5' helicases and reveal important mechanistic differences from SF1 helicases.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:17558417 Page seeded by OCA on Sun May 4 12:29:52 2008
