2pc4
From Proteopedia
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'''Crystal structure of fructose-bisphosphate aldolase from Plasmodium falciparum in complex with TRAP-tail determined at 2.4 angstrom resolution''' | '''Crystal structure of fructose-bisphosphate aldolase from Plasmodium falciparum in complex with TRAP-tail determined at 2.4 angstrom resolution''' | ||
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[[Category: Nussenzweig, V.]] | [[Category: Nussenzweig, V.]] | ||
[[Category: SGPP, Structural Genomics of Pathogenic Protozoa Consortium.]] | [[Category: SGPP, Structural Genomics of Pathogenic Protozoa Consortium.]] | ||
| - | [[Category: | + | [[Category: Aldolase]] |
| - | [[Category: | + | [[Category: Invasion machinery]] |
| - | [[Category: | + | [[Category: Plasmodium falciparum]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: Sgpp]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Structural genomics of pathogenic protozoa consortium]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:49:20 2008'' | |
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Revision as of 09:49, 4 May 2008
Crystal structure of fructose-bisphosphate aldolase from Plasmodium falciparum in complex with TRAP-tail determined at 2.4 angstrom resolution
Overview
An actomyosin motor located underneath the plasma membrane drives motility and host-cell invasion of apicomplexan parasites such as Plasmodium falciparum and Plasmodium vivax, the causative agents of malaria. Aldolase connects the motor actin filaments to transmembrane adhesive proteins of the thrombospondin-related anonymous protein (TRAP) family and transduces the motor force across the parasite surface. The TRAP-aldolase interaction is a distinctive and critical trait of host hepatocyte invasion by Plasmodium sporozoites, with a likely similar interaction crucial for erythrocyte invasion by merozoites. Here, we describe 2.4-A and 2.7-A structures of P. falciparum aldolase (PfAldo) obtained from crystals grown in the presence of the C-terminal hexapeptide of TRAP from Plasmodium berghei. The indole ring of the critical penultimate Trp-residue of TRAP fits snugly into a newly formed hydrophobic pocket, which is exclusively delimited by hydrophilic residues: two arginines, one glutamate, and one glutamine. Comparison with the unliganded PfAldo structure shows that the two arginines adopt new side-chain rotamers, whereas a 25-residue subdomain, forming a helix-loop-helix unit, shifts upon binding the TRAP-tail. The structural data are in agreement with decreased TRAP binding after mutagenesis of PfAldo residues in and near the induced TRAP-binding pocket. Remarkably, the TRAP- and actin-binding sites of PfAldo seem to overlap, suggesting that both the plasticity of the aldolase active-site region and the multimeric nature of the enzyme are crucial for its intriguing nonenzymatic function in the invasion machinery of the malaria parasite.
About this Structure
2PC4 is a Protein complex structure of sequences from Plasmodium falciparum. Full crystallographic information is available from OCA.
Reference
Aldolase provides an unusual binding site for thrombospondin-related anonymous protein in the invasion machinery of the malaria parasite., Bosch J, Buscaglia CA, Krumm B, Ingason BP, Lucas R, Roach C, Cardozo T, Nussenzweig V, Hol WG, Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):7015-20. Epub 2007 Apr 10. PMID:17426153 Page seeded by OCA on Sun May 4 12:49:20 2008
Categories: Fructose-bisphosphate aldolase | Plasmodium falciparum | Protein complex | Bosch, J. | Buscaglia, C A. | Cardozo, T. | Hol, W G.J. | Krumm, B. | Nussenzweig, V. | SGPP, Structural Genomics of Pathogenic Protozoa Consortium. | Aldolase | Invasion machinery | Protein structure initiative | Psi | Sgpp | Structural genomic | Structural genomics of pathogenic protozoa consortium
