2pc5

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[[Image:2pc5.jpg|left|200px]]
[[Image:2pc5.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2pc5 |SIZE=350|CAPTION= <scene name='initialview01'>2pc5</scene>, resolution 2.20&Aring;
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The line below this paragraph, containing "STRUCTURE_2pc5", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Residue+A+167'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= T6H20.30 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])
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-->
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|DOMAIN=
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{{STRUCTURE_2pc5| PDB=2pc5 | SCENE= }}
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|RELATEDENTRY=[[2p9o|2P9O]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pc5 OCA], [http://www.ebi.ac.uk/pdbsum/2pc5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pc5 RCSB]</span>
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}}
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'''Native crystal structure analysis on Arabidopsis dUTPase'''
'''Native crystal structure analysis on Arabidopsis dUTPase'''
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[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: dUTP diphosphatase]]
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[[Category: DUTP diphosphatase]]
[[Category: Bajaj, M.]]
[[Category: Bajaj, M.]]
[[Category: Moriyama, H.]]
[[Category: Moriyama, H.]]
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[[Category: arabidopsis]]
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[[Category: Arabidopsis]]
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[[Category: dutpase]]
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[[Category: Dutpase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: magnesium]]
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[[Category: Magnesium]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:49:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:34:08 2008''
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Revision as of 09:49, 4 May 2008

Image:2pc5.jpg

Template:STRUCTURE 2pc5

Native crystal structure analysis on Arabidopsis dUTPase


Overview

The deoxyuridine triphosphate nucleotidohydrolase gene from Arabidopsis thaliana was expressed and the gene product was purified. Crystallization was performed by the hanging-drop vapour-diffusion method at 298 K using 2 M ammonium sulfate as the precipitant. X-ray diffraction data were collected to 2.2 A resolution using Cu K alpha radiation. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 69.90, b = 70.86 A, c = 75.55 A. Assuming the presence of a trimer in the asymmetric unit, the solvent content was 30%, with a V(M) of 1.8 A3 Da(-1).

About this Structure

2PC5 is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

Reference

Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana., Bajaj M, Moriyama H, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt, 5):409-11. Epub 2007 Apr 14. PMID:17565183 Page seeded by OCA on Sun May 4 12:49:25 2008

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