2pfb
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2pfb.jpg|left|200px]] | [[Image:2pfb.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2pfb", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2pfb| PDB=2pfb | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Structure of oxidized OhrR from Xanthamonas campestris''' | '''Structure of oxidized OhrR from Xanthamonas campestris''' | ||
| Line 27: | Line 24: | ||
[[Category: Brennan, R G.]] | [[Category: Brennan, R G.]] | ||
[[Category: Newberry, K J.]] | [[Category: Newberry, K J.]] | ||
| - | [[Category: | + | [[Category: Marr family]] |
| - | [[Category: | + | [[Category: Transcription regulator]] |
| - | [[Category: | + | [[Category: Transcriptional regulator]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:59:52 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 09:59, 4 May 2008
Structure of oxidized OhrR from Xanthamonas campestris
Overview
The Xanthomonas campestris transcription regulator OhrR contains a reactive cysteine residue (C22) that upon oxidation by organic hydroperoxides (OHPs) forms an intersubunit disulphide bond with residue C127'. Such modification induces the expression of a peroxidase that reduces OHPs to their less toxic alcohols. Here, we describe the structures of reduced and OHP-oxidized OhrR, visualizing the structural mechanism of OHP induction. Reduced OhrR takes a canonical MarR family fold with C22 and C127' separated by 15.5 A. OHP oxidation results in the disruption of the Y36'-C22-Y47' interaction network and dissection of helix alpha5, which then allows the 135 degrees rotation and 8.2 A translation of C127', formation of the C22-C127' disulphide bond, and alpha6-alpha6' helix-swapped reconfiguration of the dimer interface. These changes result in the 28 degrees rigid body rotations of each winged helix-turn-helix motif and DNA dissociation. Similar effector-induced rigid body rotations are expected for most MarR family members.
About this Structure
2PFB is a Single protein structure of sequence from Xanthomonas campestris. Full crystallographic information is available from OCA.
Reference
Structural mechanism of organic hydroperoxide induction of the transcription regulator OhrR., Newberry KJ, Fuangthong M, Panmanee W, Mongkolsuk S, Brennan RG, Mol Cell. 2007 Nov 30;28(4):652-64. PMID:18042459 Page seeded by OCA on Sun May 4 12:59:52 2008
