2pfg

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[[Image:2pfg.jpg|left|200px]]
[[Image:2pfg.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2pfg |SIZE=350|CAPTION= <scene name='initialview01'>2pfg</scene>, resolution 1.54&Aring;
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The line below this paragraph, containing "STRUCTURE_2pfg", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DDD:(5R,10S)-5-{[(CARBOXYMETHYL)AMINO]CARBONYL}-7-OXO-3-THIA-1,6-DIAZABICYCLO[4.4.1]UNDECANE-10-CARBOXYLIC+ACID'>DDD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=M0H:S-(HYDROXYMETHYL)-L-CYSTEINE'>M0H</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= CBR1, CBR, CRN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_2pfg| PDB=2pfg | SCENE= }}
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|RELATEDENTRY=[[1wma|1WMA]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfg OCA], [http://www.ebi.ac.uk/pdbsum/2pfg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pfg RCSB]</span>
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}}
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'''Crystal structure of human CBR1 in complex with BiGF2.'''
'''Crystal structure of human CBR1 in complex with BiGF2.'''
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[[Category: Rauh, D.]]
[[Category: Rauh, D.]]
[[Category: Shokat, K M.]]
[[Category: Shokat, K M.]]
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[[Category: glutathione]]
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[[Category: Glutathione]]
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[[Category: macro molecule]]
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[[Category: Macro molecule]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:00:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:35:12 2008''
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Revision as of 10:00, 4 May 2008

Image:2pfg.jpg

Template:STRUCTURE 2pfg

Crystal structure of human CBR1 in complex with BiGF2.


Overview

Glutathione forms complex reaction products with formaldehyde, which can be further modified through enzymatic modification. We studied the non-enzymatic reaction between glutathione and formaldehyde and identified a bicyclic complex containing two equivalents of formaldehyde and one glutathione molecule by protein X-ray crystallography (PDB accession number 2PFG). We have also used (1)H, (13)C and 2D NMR spectroscopy to confirm the structure of this unusual adduct. The key feature of this adduct is the involvement of the gamma-glutamyl alpha-amine and the Cys thiol in the formation of the bicyclic ring structure. These findings suggest that the structure of GSH allows for bi-dentate masking of the reactivity of formaldehyde. As this species predominates at near physiological pH values, we suggest this adduct may have biological significance.

About this Structure

2PFG is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Glutathione traps formaldehyde by formation of a bicyclo[4.4.1]undecane adduct., Bateman R, Rauh D, Shokat KM, Org Biomol Chem. 2007 Oct 21;5(20):3363-7. Epub 2007 Aug 29. PMID:17912391 Page seeded by OCA on Sun May 4 13:00:15 2008

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OCA

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