2pgg
From Proteopedia
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'''Crystal Structure of a Birnavirus (IBDV) RNA-dependent RNA Polymerase VP1''' | '''Crystal Structure of a Birnavirus (IBDV) RNA-dependent RNA Polymerase VP1''' | ||
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[[Category: Tao, Y J.]] | [[Category: Tao, Y J.]] | ||
[[Category: Vakharia, V N.]] | [[Category: Vakharia, V N.]] | ||
| - | [[Category: | + | [[Category: Birnavirus]] |
| - | [[Category: | + | [[Category: Dsrna virus]] |
| - | [[Category: | + | [[Category: Ibdv]] |
| - | [[Category: | + | [[Category: Permutation]] |
| - | [[Category: | + | [[Category: Polymerase]] |
| - | [[Category: | + | [[Category: Rdrp]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:03:47 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:03, 4 May 2008
Crystal Structure of a Birnavirus (IBDV) RNA-dependent RNA Polymerase VP1
Overview
Single-subunit polymerases are universally encoded in both cellular organisms and viruses. Their three-dimensional structures have the shape of a right-hand with the active site located in the palm region, which has a topology similar to that of the RNA recognition motif (RRM) found in many RNA-binding proteins. Considering that polymerases have well conserved structures, it was surprising that the RNA-dependent RNA polymerases from birnaviruses, a group of dsRNA viruses, have their catalytic motifs arranged in a permuted order in sequence. Here we report the 2.5 A structure of a birnavirus VP1 in which the polymerase palm subdomain adopts a new active site topology that has not been previously observed in other polymerases. In addition, the polymerase motif C of VP1 has the sequence of -ADN-, a highly unusual feature for RNA-dependent polymerases. Through site-directed mutagenesis, we have shown that changing the VP1 motif C from -ADN- to -GDD- results in a mutant with an increased RNA synthesis activity. Our results indicate that the active site topology of VP1 may represent a newly developed branch in polymerase evolution, and that birnaviruses may have acquired the -ADN- mutation to control their growth rate.
About this Structure
2PGG is a Single protein structure of sequence from Infectious bursal disease virus. Full crystallographic information is available from OCA.
Reference
The structure of a birnavirus polymerase reveals a distinct active site topology., Pan J, Vakharia VN, Tao YJ, Proc Natl Acad Sci U S A. 2007 May 1;104(18):7385-90. Epub 2007 Apr 24. PMID:17456597 Page seeded by OCA on Sun May 4 13:03:47 2008
