2pjh

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[[Image:2pjh.jpg|left|200px]]
[[Image:2pjh.jpg|left|200px]]
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{{Structure
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|PDB= 2pjh |SIZE=350|CAPTION= <scene name='initialview01'>2pjh</scene>
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The line below this paragraph, containing "STRUCTURE_2pjh", creates the "Structure Box" on the page.
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|SITE=
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|GENE= p97 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), npl4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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{{STRUCTURE_2pjh| PDB=2pjh | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pjh OCA], [http://www.ebi.ac.uk/pdbsum/2pjh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pjh RCSB]</span>
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'''Strctural Model of the p97 N domain- npl4 UBD complex'''
'''Strctural Model of the p97 N domain- npl4 UBD complex'''
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[[Category: Simpson, S.]]
[[Category: Simpson, S.]]
[[Category: Zhang, X.]]
[[Category: Zhang, X.]]
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[[Category: aaa]]
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[[Category: Aaa]]
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[[Category: atpase]]
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[[Category: Atpase]]
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[[Category: npl4]]
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[[Category: Npl4]]
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[[Category: p97]]
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[[Category: P97]]
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[[Category: protein binding]]
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[[Category: Protein binding]]
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[[Category: transport protein]]
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[[Category: Transport protein]]
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[[Category: ufd1]]
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[[Category: Ufd1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:14:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:36:44 2008''
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Revision as of 10:14, 4 May 2008

Image:2pjh.jpg

Template:STRUCTURE 2pjh

Strctural Model of the p97 N domain- npl4 UBD complex


Overview

The AAA ATPase, p97, achieves its versatility through binding to a wide range of cofactor proteins that adapt it to different cellular functions. The heterodimer UN (comprising Ufd1 and Npl4) is an adaptor complex that recruits p97 for numerous tasks, many of which involve the ubiquitin pathway. Insights into the structural specificity of p97 for its UN adaptor are currently negligible. Here, we present the solution structure of the Npl4 "ubiquitin-like" domain (UBD), which adopts a beta-grasp fold with a 3(10) helical insert. Moreover we performed a chemical shift perturbation analysis of its binding surface with the p97 N domain. We assigned the backbone amides of the p97 N domain and probed both its reciprocal binding surface with Npl4 UBD and its interaction with the p97-binding region of Ufd1. NMR data recorded on a 400-kDa full-length UN-hexamer p97 complex reveals an identical mode of interaction. We calculated a structural model for the p97 N-Npl4 UBD complex, and a comparison with the p97-p47 adaptor complex reveals subtle differences in p97 adaptor recognition and specificity.

About this Structure

2PJH is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Detailed structural insights into the p97-Npl4-Ufd1 interface., Isaacson RL, Pye VE, Simpson P, Meyer HH, Zhang X, Freemont PS, Matthews S, J Biol Chem. 2007 Jul 20;282(29):21361-9. Epub 2007 May 9. PMID:17491009 Page seeded by OCA on Sun May 4 13:14:11 2008

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