2pkc

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[[Image:2pkc.jpg|left|200px]]
[[Image:2pkc.jpg|left|200px]]
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{{Structure
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|PDB= 2pkc |SIZE=350|CAPTION= <scene name='initialview01'>2pkc</scene>, resolution 1.5&Aring;
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The line below this paragraph, containing "STRUCTURE_2pkc", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span>
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{{STRUCTURE_2pkc| PDB=2pkc | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pkc OCA], [http://www.ebi.ac.uk/pdbsum/2pkc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pkc RCSB]</span>
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'''CRYSTAL STRUCTURE OF CALCIUM-FREE PROTEINASE K AT 1.5 ANGSTROMS RESOLUTION'''
'''CRYSTAL STRUCTURE OF CALCIUM-FREE PROTEINASE K AT 1.5 ANGSTROMS RESOLUTION'''
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[[Category: Saenger, W.]]
[[Category: Saenger, W.]]
[[Category: Wolf, W M.]]
[[Category: Wolf, W M.]]
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[[Category: hydrolase(serine proteinase)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:17:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:37:11 2008''
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Revision as of 10:17, 4 May 2008

Image:2pkc.jpg

Template:STRUCTURE 2pkc

CRYSTAL STRUCTURE OF CALCIUM-FREE PROTEINASE K AT 1.5 ANGSTROMS RESOLUTION


Overview

Proteinase K from the fungus Tritirachium album Limber binds two Ca2+ ions, one strongly (Ca 1) and the other weakly (Ca 2). Removal of these cations reduces the stability of proteinase K as shown by thermal denaturation, but the proteolytic activity is unchanged. The x-ray structures of native and Ca(2+)-free proteinase K at 1.5-A resolution show that there are no cuts in the polypeptide backbone (i.e. no autolysis), Ca 1 has been replaced by Na+, while Ca 2 has been substituted by a water associated with a larger but locally confined structural change at that site. A small but concerted geometrical shift is transmitted from the Ca 1 site via eight secondary structure elements to the substrate recognition site (Gly100-Tyr104, and Ser132-Gly136) but not to the catalytic triad (Asp39,His69,Ser224). This is accompanied by positional changes of localized waters.

About this Structure

2PKC is a Single protein structure of sequence from Engyodontium album. Full crystallographic information is available from OCA.

Reference

Crystal structure of calcium-free proteinase K at 1.5-A resolution., Muller A, Hinrichs W, Wolf WM, Saenger W, J Biol Chem. 1994 Sep 16;269(37):23108-11. PMID:8083213 Page seeded by OCA on Sun May 4 13:17:28 2008

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