This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2pnh
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2pnh.jpg|left|200px]] | [[Image:2pnh.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2pnh", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2pnh| PDB=2pnh | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''Escherichia coli PriB E39A variant''' | '''Escherichia coli PriB E39A variant''' | ||
| Line 27: | Line 24: | ||
[[Category: Keck, J L.]] | [[Category: Keck, J L.]] | ||
[[Category: Lopper, M E.]] | [[Category: Lopper, M E.]] | ||
| - | [[Category: | + | [[Category: Beta barrel]] |
| - | [[Category: | + | [[Category: Dna binding protein]] |
| - | [[Category: | + | [[Category: Ob fold]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:28:49 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:28, 4 May 2008
Escherichia coli PriB E39A variant
Overview
Collapsed DNA replication forks must be reactivated through origin-independent reloading of the replication machinery (replisome) to ensure complete duplication of cellular genomes. In E. coli, the PriA-dependent pathway is the major replication restart mechanism and requires primosome proteins PriA, PriB, and DnaT for replisome reloading. However, the molecular mechanisms that regulate origin-independent replisome loading are not fully understood. Here, we demonstrate that assembly of primosome protein complexes represents a key regulatory mechanism, as inherently weak PriA-PriB and PriB-DnaT interactions are strongly stimulated by single-stranded DNA. Furthermore, the binding site on PriB for single-stranded DNA partially overlaps the binding sites for PriA and DnaT, suggesting a dynamic primosome assembly process in which single-stranded DNA is handed off from one primosome protein to another as a repaired replication fork is reactivated. This model helps explain how origin-independent initiation of DNA replication is restricted to repaired replication forks, preventing overreplication of the genome.
About this Structure
2PNH is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A hand-off mechanism for primosome assembly in replication restart., Lopper M, Boonsombat R, Sandler SJ, Keck JL, Mol Cell. 2007 Jun 22;26(6):781-93. PMID:17588514 Page seeded by OCA on Sun May 4 13:28:49 2008
