2poo

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[[Image:2poo.jpg|left|200px]]
[[Image:2poo.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2poo |SIZE=350|CAPTION= <scene name='initialview01'>2poo</scene>, resolution 2.050&Aring;
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The line below this paragraph, containing "STRUCTURE_2poo", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8] </span>
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|GENE=
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{{STRUCTURE_2poo| PDB=2poo | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2poo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2poo OCA], [http://www.ebi.ac.uk/pdbsum/2poo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2poo RCSB]</span>
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}}
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'''THERMOSTABLE PHYTASE IN FULLY CALCIUM LOADED STATE'''
'''THERMOSTABLE PHYTASE IN FULLY CALCIUM LOADED STATE'''
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[[Category: Ha, N C.]]
[[Category: Ha, N C.]]
[[Category: Oh, B H.]]
[[Category: Oh, B H.]]
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[[Category: calcium loaded state]]
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[[Category: Calcium loaded state]]
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[[Category: thermostable phytase]]
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[[Category: Thermostable phytase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:32:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:38:48 2008''
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Revision as of 10:33, 4 May 2008

Image:2poo.jpg

Template:STRUCTURE 2poo

THERMOSTABLE PHYTASE IN FULLY CALCIUM LOADED STATE


Overview

A novel bacterial phytase from a Bacillus amyloliquefaciens strain was crystallized using the hanging-drop vapour-diffusion method. The amino-acid sequence of the enzyme does not show any homology to those of other known phytases or phosphatases, with the exception of a phytase from Bacillus subtilis. The enzyme exhibits a thermal stability which is strongly dependent on calcium ions. High-quality single crystals of the enzyme in the absence of calcium ions were obtained using a precipitant solution containing 20% 2-methyl-2, 4-pentanediol and 0.1 M MES (pH 6.5). Native diffraction data to 2.0 A resolution were obtained from a flash-frozen crystal at 110 K using a rotating-anode X-ray source. The crystals belong to space group P212121 with unit-cell dimensions a = 50.4, b = 64.1, c = 104. 2 A and contain one monomer per asymmetric unit. Structure determination using heavy-atom derivative crystals is in progress, along with an effort to crystallize the calcium ion bound form of the enzyme.

About this Structure

2POO is a Single protein structure of sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA.

Reference

Preliminary X-ray crystallographic analysis of a novel phytase from a Bacillus amyloliquefaciens strain., Ha NC, Kim YO, Oh TK, Oh BH, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):691-3. PMID:10089471 Page seeded by OCA on Sun May 4 13:32:58 2008

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