2pox
From Proteopedia
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'''Dark state structure of the reversibly switchable fluorescent protein Dronpa''' | '''Dark state structure of the reversibly switchable fluorescent protein Dronpa''' | ||
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[[Category: Wahl, M C.]] | [[Category: Wahl, M C.]] | ||
[[Category: Weber, G.]] | [[Category: Weber, G.]] | ||
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| - | [[Category: | + | [[Category: Luminescent protein]] |
| - | [[Category: | + | [[Category: Reversibly switchable fluorescent protein]] |
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Revision as of 10:33, 4 May 2008
Dark state structure of the reversibly switchable fluorescent protein Dronpa
Overview
Dronpa is a novel GFP-like fluorescent protein with exceptional light-controlled switching properties. It may be reversibly switched between a fluorescent on-state and a nonfluorescent off-state by irradiation with light. To elucidate the molecular basis of the switching mechanism, we generated reversibly switchable Dronpa protein crystals. Using these crystals we determined the elusive dark-state structure of Dronpa at 1.95-A resolution. We found that the photoswitching results in a cis-trans isomerization of the chromophore accompanied by complex structural rearrangements of four nearby amino acid residues. Because of this cascade of intramolecular events, the chromophore is exposed to distinct electrostatic surface potentials, which are likely to influence the protonation equilibria at the chromophore. We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore.
About this Structure
2POX is a Single protein structure of sequence from Echinophyllia sp. sc22. Full crystallographic information is available from OCA.
Reference
Structural basis for reversible photoswitching in Dronpa., Andresen M, Stiel AC, Trowitzsch S, Weber G, Eggeling C, Wahl MC, Hell SW, Jakobs S, Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):13005-9. Epub 2007 Jul 23. PMID:17646653 Page seeded by OCA on Sun May 4 13:33:44 2008
