1nsn
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(New page: 200px<br /> <applet load="1nsn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nsn, resolution 2.8Å" /> '''THE CRYSTAL STRUCTUR...)
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Revision as of 07:31, 18 November 2007
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THE CRYSTAL STRUCTURE OF ANTIBODY N10-STAPHYLOCOCCAL NUCLEASE COMPLEX AT 2.9 ANGSTROMS RESOLUTION
Overview
The three-dimensional structure of the antibody N10 Fab fragment complexed, with staphylococcal nuclease (SNase) has been determined to 2.9 A, resolution. Eighteen residues from six complementarity-determining regions, (CDR) recognize an epitope of five distinct SNase segments with a total of, 17 residues. The overall shape of the antibody-antigen interface is, U-shaped rather than the more or less rectangular interface seen in other, antibody-protein antigen interfaces. Despite the U-shaped interface, the, amount of surface buried in the complex, 828 A2 for SNase and 793 A2 for, N10, is typical of antibody-protein antigen complexes. Contributing to the, shape of the interface is the shortest antibody heavy chain-CDR3 loop, reported to date, which probably allows access of bulk solvent in the, center of the "U" interface. Another unusual feature of the N10 antibody, is the 15 residue antibody light chain-CDR1, a length seen in only three, other reported antibodies. Antibody light chain-CDR1 displays a previously, unobserved conformation in its distal portion. Finally, although some of, the movement observed in the antibody-bound SNase may be due to crystal, contacts, it is clear that some side-chain rearrangements are the result, of antigen-antibody interaction.
About this Structure
1NSN is a Protein complex structure of sequences from Mus musculus and Staphylococcus aureus. Active as Micrococcal nuclease, with EC number 3.1.31.1 Full crystallographic information is available from OCA.
Reference
The crystal structure of the antibody N10-staphylococcal nuclease complex at 2.9 A resolution., Bossart-Whitaker P, Chang CY, Novotny J, Benjamin DC, Sheriff S, J Mol Biol. 1995 Nov 3;253(4):559-75. PMID:7473734
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