2pt7
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2pt7.jpg|left|200px]] | [[Image:2pt7.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2pt7", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2pt7| PDB=2pt7 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal structure of Cag VirB11 (HP0525) and an inhibitory protein (HP1451)''' | '''Crystal structure of Cag VirB11 (HP0525) and an inhibitory protein (HP1451)''' | ||
| Line 32: | Line 29: | ||
[[Category: Waksman, G.]] | [[Category: Waksman, G.]] | ||
[[Category: Williams, R.]] | [[Category: Williams, R.]] | ||
| - | [[Category: | + | [[Category: Atpase]] |
| - | [[Category: | + | [[Category: Hydrolase/protein binding complex]] |
| - | [[Category: | + | [[Category: Protein-protein complex]] |
| - | [[Category: | + | [[Category: Type iv secretion]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:46:11 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:46, 4 May 2008
Crystal structure of Cag VirB11 (HP0525) and an inhibitory protein (HP1451)
Overview
Helicobacter pylori is one of the world's most successful human pathogens causing gastric ulcers and cancers. A key virulence factor of H. pylori is the Cag pathogenicity island, which encodes a type IV secretion system. HP0525 is an essential component of the Cag system and acts as an inner membrane associated ATPase. HP0525 forms double hexameric ring structures, with the C-terminal domains (CTDs) forming a closed ring and the N-terminal domains (NTDs) forming a dynamic, open ring. Here, the crystal structure of HP0525 in complex with a fragment of HP1451, a protein of previously unknown function, is reported. The HP1451 construct consists of two domains similar to nucleic acid-binding domains. Two HP1451 molecules bind to the HP0525 NTDs on opposite sides of the hexamer, locking it in the closed form and forming a partial lid over the HP0525 chamber. From the structure, it is suggested that HP1451 acts as an inhibitory factor of HP0525 to regulate Cag-mediated secretion, a suggestion confirmed by results of in vitro ATPase assay and in vivo pull-down experiments.
About this Structure
2PT7 is a Protein complex structure of sequences from Helicobacter pylori. Full crystallographic information is available from OCA.
Reference
Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase., Hare S, Fischer W, Williams R, Terradot L, Bayliss R, Haas R, Waksman G, EMBO J. 2007 Nov 28;26(23):4926-34. Epub 2007 Nov 1. PMID:17972918 Page seeded by OCA on Sun May 4 13:46:11 2008
