1psk
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(New page: 200px<br /> <applet load="1psk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1psk, resolution 2.8Å" /> '''THE CRYSTAL STRUCTUR...)
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Revision as of 07:32, 18 November 2007
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THE CRYSTAL STRUCTURE OF AN FAB FRAGMENT THAT BINDS TO THE MELANOMA-ASSOCIATED GD2 GANGLIOSIDE
Overview
The GD2 ganglioside is a cell-surface component that appears on the, surface of metastatic melanoma cells and is a marker for the progression, of the disease. The ME36.1 monoclonal antibody binds to the GD2, ganglioside and has shown potential as a therapeutic antibody. ME36.1 is a, possible alternative therapy to radiation, which is often ineffective in, late-stage melanoma. The crystal structure of the Fab fragment of ME36.1, has been determined using molecular replacement and refined to an R factor, of 20.4% at 2.8 A resolution. The model has good geometry with, root-mean-square deviations of 0.008 A from ideal bond lengths and 1.7, degrees from ideal bond angles. The crystal structure of the ME36.1 Fab, shows that its complementarity determining region forms a groove-shaped, binding site rather than the pocket-type observed in other sugar binding, Fabs. Molecular modeling has placed a four-residue sugar, representative, of GD2, in the antigen binding site. The GD2 sugar moiety is stabilized by, a network of hydrogen bonds that define the specificity of ME36.1 toward, its antigen.
About this Structure
1PSK is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
The crystal structure of a Fab fragment to the melanoma-associated GD2 ganglioside., Pichla SL, Murali R, Burnett RM, J Struct Biol. 1997 Jun;119(1):6-16. PMID:9216084
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