2pvi

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[[Image:2pvi.gif|left|200px]]
[[Image:2pvi.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2pvi |SIZE=350|CAPTION= <scene name='initialview01'>2pvi</scene>, resolution 1.76&Aring;
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The line below this paragraph, containing "STRUCTURE_2pvi", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=I5C:5-IODO-2&#39;-DEOXY-CYTIDINE-5&#39;-MONOPHOSPHATE'>I5C</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2pvi| PDB=2pvi | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pvi OCA], [http://www.ebi.ac.uk/pdbsum/2pvi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pvi RCSB]</span>
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}}
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'''PVUII ENDONUCLEASE COMPLEXED TO AN IODINATED COGNATE DNA'''
'''PVUII ENDONUCLEASE COMPLEXED TO AN IODINATED COGNATE DNA'''
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[[Category: Cheng, X.]]
[[Category: Cheng, X.]]
[[Category: Horton, J.]]
[[Category: Horton, J.]]
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[[Category: complex (restriction endonuclease/dna)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:52:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:41:18 2008''
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Revision as of 10:52, 4 May 2008

Image:2pvi.gif

Template:STRUCTURE 2pvi

PVUII ENDONUCLEASE COMPLEXED TO AN IODINATED COGNATE DNA


Overview

In restriction-modification systems, cleavage of substrate sites in cellular DNA by the restriction endonuclease is prevented by the action of a cognate methyltransferase that acts on the same substrate sites. The PvuII restriction endonuclease (R.PvuII) has been structurally characterized in a complex with substrate DNA (Cheng et al., 1994) and as an apoenzyme (Athanasiadis et al., 1994). We report here a structure, determined to 1.9 A resolution by crystallography, of a complex between R.PvuII and iodinated DNA. The presence of an iodine at the 5-carbon of the methylatable cytosine results in the following changes in the protein: His84 moved away from the modified base; this movement was amplified in His85 and disrupts an intersubunit hydrogen bond; and the base modification disturbs the distribution of water molecules that associate with these histidine residues and the area of the scissile bond. Considering these observations, hypotheses are given as to why a similar oligonucleotide, where a methyl group resides on the 5-carbon of the methylatable cytosine, is slowly cleaved by R.PvuII (Rice et al., 1995).

About this Structure

2PVI is a Single protein structure of sequence from Proteus vulgaris. Full crystallographic information is available from OCA.

Reference

How is modification of the DNA substrate recognized by the PvuII restriction endonuclease?, Horton JR, Bonventre J, Cheng X, Biol Chem. 1998 Apr-May;379(4-5):451-8. PMID:9628337 Page seeded by OCA on Sun May 4 13:52:13 2008

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