2pz9

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[[Image:2pz9.jpg|left|200px]]
[[Image:2pz9.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2pz9 |SIZE=350|CAPTION= <scene name='initialview01'>2pz9</scene>, resolution 2.80&Aring;
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The line below this paragraph, containing "STRUCTURE_2pz9", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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|GENE= SCO4942, 2SCK31.02c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria])
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG1309 AcrR], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam00440 TetR_N]</span>
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{{STRUCTURE_2pz9| PDB=2pz9 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pz9 OCA], [http://www.ebi.ac.uk/pdbsum/2pz9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pz9 RCSB]</span>
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'''Crystal structure of putative transcriptional regulator SCO4942 from Streptomyces coelicolor'''
'''Crystal structure of putative transcriptional regulator SCO4942 from Streptomyces coelicolor'''
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[[Category: Xu, X.]]
[[Category: Xu, X.]]
[[Category: Zheng, H.]]
[[Category: Zheng, H.]]
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[[Category: mcsg]]
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[[Category: Mcsg]]
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[[Category: midwest center for structural genomic]]
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[[Category: Midwest center for structural genomic]]
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[[Category: protein structure initiative]]
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[[Category: Protein structure initiative]]
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[[Category: psi]]
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[[Category: Psi]]
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[[Category: streptomyces coelicolor a3(2)]]
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[[Category: Structural genomic]]
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[[Category: structural genomic]]
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[[Category: Transcriptional regulator]]
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[[Category: transcriptional regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:03:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:42:36 2008''
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Revision as of 11:03, 4 May 2008

Image:2pz9.jpg

Template:STRUCTURE 2pz9

Crystal structure of putative transcriptional regulator SCO4942 from Streptomyces coelicolor


Overview

We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.

About this Structure

2PZ9 is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.

Reference

In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461 Page seeded by OCA on Sun May 4 14:03:09 2008

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