2q0q

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2q0q.jpg|left|200px]]
[[Image:2q0q.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2q0q |SIZE=350|CAPTION= <scene name='initialview01'>2q0q</scene>, resolution 1.50&Aring;
+
The line below this paragraph, containing "STRUCTURE_2q0q", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Arylesterase Arylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.2 3.1.1.2] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2q0q| PDB=2q0q | SCENE= }}
-
|RELATEDENTRY=[[2q0s|2q0s]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q0q OCA], [http://www.ebi.ac.uk/pdbsum/2q0q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2q0q RCSB]</span>
+
-
}}
+
'''Structure of the Native M. Smegmatis Aryl Esterase'''
'''Structure of the Native M. Smegmatis Aryl Esterase'''
Line 35: Line 32:
[[Category: Weyler, W.]]
[[Category: Weyler, W.]]
[[Category: Whited, G.]]
[[Category: Whited, G.]]
-
[[Category: acyl transfer]]
+
[[Category: Acyl transfer]]
-
[[Category: aryl esterase]]
+
[[Category: Aryl esterase]]
-
[[Category: oligomeric enzyme]]
+
[[Category: Oligomeric enzyme]]
-
[[Category: sgnh hydrolase]]
+
[[Category: Sgnh hydrolase]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:07:50 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:43:13 2008''
+

Revision as of 11:07, 4 May 2008

Image:2q0q.jpg

Template:STRUCTURE 2q0q

Structure of the Native M. Smegmatis Aryl Esterase


Overview

The unusual architecture of the enzyme (MsAcT) isolated from Mycobacterium smegmatis forms the mechanistic basis for favoring alcoholysis over hydrolysis in water. Unlike hydrolases that perform alcoholysis only under anhydrous conditions, MsAcT demonstrates alcoholysis in substantially aqueous media and, in the presence of hydrogen peroxide, has a perhydrolysis:hydrolysis ratio 50-fold greater than that of the best lipase tested. The crystal structures of the apoenzyme and an inhibitor-bound form have been determined to 1.5 A resolution. MsAcT is an octamer in the asymmetric unit and forms a tightly associated aggregate in solution. Relative to other structurally similar monomers, MsAcT contains several insertions that contribute to the oligomerization and greatly restrict the shape of the active site, thereby limiting its accessibility. These properties create an environment by which MsAcT can catalyze transesterification reactions in an aqueous medium and suggests how a serine hydrolase can be engineered to be an efficient acyltransferase.

About this Structure

2Q0Q is a Single protein structure of sequence from Mycobacterium smegmatis. Full crystallographic information is available from OCA.

Reference

Structure of a novel enzyme that catalyzes acyl transfer to alcohols in aqueous conditions., Mathews I, Soltis M, Saldajeno M, Ganshaw G, Sala R, Weyler W, Cervin MA, Whited G, Bott R, Biochemistry. 2007 Aug 7;46(31):8969-79. Epub 2007 Jul 18. PMID:17636869 Page seeded by OCA on Sun May 4 14:07:50 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2q0q"
Personal tools