2q2t

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[[Image:2q2t.gif|left|200px]]
[[Image:2q2t.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2q2t |SIZE=350|CAPTION= <scene name='initialview01'>2q2t</scene>, resolution 2.300&Aring;
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The line below this paragraph, containing "STRUCTURE_2q2t", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=OMC:O2&#39;-METHYLYCYTIDINE-5&#39;-MONOPHOSPHATE'>OMC</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= A544R ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Paramecium bursaria Chlorella virus 1])
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-->
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|DOMAIN=
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{{STRUCTURE_2q2t| PDB=2q2t | SCENE= }}
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|RELATEDENTRY=[[1fvi|1FVI]], [[2q2u|2Q2U]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q2t OCA], [http://www.ebi.ac.uk/pdbsum/2q2t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2q2t RCSB]</span>
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}}
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'''Structure of Chlorella virus DNA ligase-adenylate bound to a 5' phosphorylated nick'''
'''Structure of Chlorella virus DNA ligase-adenylate bound to a 5' phosphorylated nick'''
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[[Category: Shuman, S.]]
[[Category: Shuman, S.]]
[[Category: Smith, P.]]
[[Category: Smith, P.]]
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[[Category: ligase]]
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[[Category: Ligase]]
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[[Category: ligase/dna complex]]
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[[Category: Ligase/dna complex]]
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[[Category: lysine adenylate]]
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[[Category: Lysine adenylate]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:13:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:43:48 2008''
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Revision as of 11:13, 4 May 2008

Image:2q2t.gif

Template:STRUCTURE 2q2t

Structure of Chlorella virus DNA ligase-adenylate bound to a 5' phosphorylated nick


Overview

Chlorella virus DNA ligase, the smallest eukaryotic ligase known, has pluripotent biological activity and an intrinsic nick-sensing function, despite having none of the accessory domains found in cellular ligases. A 2.3-A crystal structure of the Chlorella virus ligase-AMP intermediate bound to duplex DNA containing a 3'-OH-5'-PO4 nick reveals a new mode of DNA envelopment, in which a short surface loop emanating from the OB domain forms a beta-hairpin 'latch' that inserts into the DNA major groove flanking the nick. A network of interactions with the 3'-OH and 5'-PO4 termini in the active site illuminates the DNA adenylylation mechanism and the crucial roles of AMP in nick sensing and catalysis. Addition of a divalent cation triggered nick sealing in crystallo, establishing that the nick complex is a bona fide intermediate in the DNA repair pathway.

About this Structure

2Q2T is a Single protein structure of sequence from Paramecium bursaria chlorella virus 1. Full crystallographic information is available from OCA.

Reference

Structural basis for nick recognition by a minimal pluripotent DNA ligase., Nair PA, Nandakumar J, Smith P, Odell M, Lima CD, Shuman S, Nat Struct Mol Biol. 2007 Aug;14(8):770-8. Epub 2007 Jul 8. PMID:17618295 Page seeded by OCA on Sun May 4 14:13:20 2008

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OCA

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