2q6l

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[[Image:2q6l.jpg|left|200px]]
[[Image:2q6l.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2q6l |SIZE=350|CAPTION= <scene name='initialview01'>2q6l</scene>, resolution 2.72&Aring;
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The line below this paragraph, containing "STRUCTURE_2q6l", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=5CD:5&#39;-CHLORO-5&#39;-DEOXYADENOSINE'>5CD</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= salL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria])
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-->
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|DOMAIN=
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{{STRUCTURE_2q6l| PDB=2q6l | SCENE= }}
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|RELATEDENTRY=[[2q6o|2Q6O]], [[2q6k|2Q6K]], [[2q6i|2Q6I]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6l OCA], [http://www.ebi.ac.uk/pdbsum/2q6l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2q6l RCSB]</span>
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}}
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'''SalL double mutant Y70T/G131S with CLDA and L-MET'''
'''SalL double mutant Y70T/G131S with CLDA and L-MET'''
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[[Category: Noel, J P.]]
[[Category: Noel, J P.]]
[[Category: Pojer, F.]]
[[Category: Pojer, F.]]
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[[Category: biosynthetic protein]]
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[[Category: Biosynthetic protein]]
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[[Category: chlorinase]]
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[[Category: Chlorinase]]
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[[Category: double mutant complex with clda and l-met]]
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[[Category: Double mutant complex with clda and l-met]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:26:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:45:26 2008''
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Revision as of 11:26, 4 May 2008

Image:2q6l.jpg

Template:STRUCTURE 2q6l

SalL double mutant Y70T/G131S with CLDA and L-MET


Overview

Halogen atom incorporation into a scaffold of bioactive compounds often amplifies biological activity, as is the case for the anticancer agent salinosporamide A (1), a chlorinated natural product from the marine bacterium Salinispora tropica. Significant effort in understanding enzymatic chlorination shows that oxidative routes predominate to form reactive electrophilic or radical chlorine species. Here we report the genetic, biochemical and structural characterization of the chlorinase SalL, which halogenates S-adenosyl-L-methionine (2) with chloride to generate 5'-chloro-5'-deoxyadenosine (3) and L-methionine (4) in a rarely observed nucleophilic substitution strategy analogous to that of Streptomyces cattleya fluorinase. Further metabolic tailoring produces a halogenated polyketide synthase substrate specific for salinosporamide A biosynthesis. SalL also accepts bromide and iodide as substrates, but not fluoride. High-resolution crystal structures of SalL and active site mutants complexed with substrates and products support the S(N)2 nucleophilic substitution mechanism and further illuminate halide specificity in this newly discovered halogenase family.

About this Structure

2Q6L is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.

Reference

Discovery and characterization of a marine bacterial SAM-dependent chlorinase., Eustaquio AS, Pojer F, Noel JP, Moore BS, Nat Chem Biol. 2008 Jan;4(1):69-74. Epub 2007 Dec 2. PMID:18059261 Page seeded by OCA on Sun May 4 14:26:48 2008

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