2qa2
From Proteopedia
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'''Crystal structure of CabE, an aromatic hydroxylase from angucycline biosynthesis, determined to 2.7 A resolution''' | '''Crystal structure of CabE, an aromatic hydroxylase from angucycline biosynthesis, determined to 2.7 A resolution''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QA2 OCA]. | |
==Reference== | ==Reference== | ||
Crystal structures of two aromatic hydroxylases involved in the early tailoring steps of angucycline biosynthesis., Koskiniemi H, Metsa-Ketela M, Dobritzsch D, Kallio P, Korhonen H, Mantsala P, Schneider G, Niemi J, J Mol Biol. 2007 Sep 21;372(3):633-48. Epub 2007 Jul 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17669423 17669423] | Crystal structures of two aromatic hydroxylases involved in the early tailoring steps of angucycline biosynthesis., Koskiniemi H, Metsa-Ketela M, Dobritzsch D, Kallio P, Korhonen H, Mantsala P, Schneider G, Niemi J, J Mol Biol. 2007 Sep 21;372(3):633-48. Epub 2007 Jul 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17669423 17669423] | ||
| - | [[Category: Protein complex]] | ||
| - | [[Category: Streptomyces sp. 431h01]] | ||
[[Category: Dobritzsch, D.]] | [[Category: Dobritzsch, D.]] | ||
[[Category: Kallio, P.]] | [[Category: Kallio, P.]] | ||
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[[Category: Niemi, J.]] | [[Category: Niemi, J.]] | ||
[[Category: Schneider, G.]] | [[Category: Schneider, G.]] | ||
| - | [[Category: | + | [[Category: Angucycline]] |
| - | [[Category: | + | [[Category: Aromatic hydroxylase]] |
| - | [[Category: | + | [[Category: Fad]] |
| - | [[Category: | + | [[Category: Oxidoreductase]] |
| - | [[Category: | + | [[Category: Polyketide]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:36:39 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:36, 4 May 2008
Crystal structure of CabE, an aromatic hydroxylase from angucycline biosynthesis, determined to 2.7 A resolution
Overview
Angucyclines are aromatic polyketides produced in Streptomycetes via complex enzymatic biosynthetic pathways. PgaE and CabE from S. sp PGA64 and S. sp. H021 are two related homo-dimeric FAD and NADPH dependent aromatic hydroxylases involved in the early steps of the angucycline core modification. Here we report the three-dimensional structures of these two enzymes determined by X-ray crystallography using multiple anomalous diffraction and molecular replacement, respectively, to resolutions of 1.8 A and 2.7 A. The enzyme subunits are built up of three domains, a FAD binding domain, a domain involved in substrate binding and a C-terminal thioredoxin-like domain of unknown function. The structure analysis identifies PgaE and CabE as members of the para-hydroxybenzoate hydroxylase (pHBH) fold family of aromatic hydroxylases. In contrast to phenol hydroxylase and 3-hydroxybenzoate hydroxylase that utilize the C-terminal domain for dimer formation, this domain is not part of the subunit-subunit interface in PgaE and CabE. Instead, dimer assembly occurs through interactions of their FAD binding domains. FAD is bound non-covalently in the "in"-conformation. The active sites in the two enzymes differ significantly from those of other aromatic hydroxylases. The volumes of the active site are significantly larger, as expected in view of the voluminous tetracyclic angucycline substrates. The structures further suggest that substrate binding and catalysis may involve dynamic rearrangements of the middle domain relative to the other two domains. Site-directed mutagenesis studies of putative catalytic groups in the active site of PgaE argue against enzyme-catalyzed substrate deprotonation as a step in catalysis. This is in contrast to pHBH, where deprotonation/protonation of the substrate has been suggested as an essential part of the enzymatic mechanism.
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal structures of two aromatic hydroxylases involved in the early tailoring steps of angucycline biosynthesis., Koskiniemi H, Metsa-Ketela M, Dobritzsch D, Kallio P, Korhonen H, Mantsala P, Schneider G, Niemi J, J Mol Biol. 2007 Sep 21;372(3):633-48. Epub 2007 Jul 10. PMID:17669423 Page seeded by OCA on Sun May 4 14:36:39 2008
