This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2qij
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2qij.jpg|left|200px]] | [[Image:2qij.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2qij", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2qij| PDB=2qij | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Hepatitis B Capsid Protein with an N-terminal extension modelled into 8.9 A data.''' | '''Hepatitis B Capsid Protein with an N-terminal extension modelled into 8.9 A data.''' | ||
| Line 29: | Line 26: | ||
[[Category: Tan, W S]] | [[Category: Tan, W S]] | ||
[[Category: Walkinshaw, M D.]] | [[Category: Walkinshaw, M D.]] | ||
| - | [[Category: | + | [[Category: Icosahedral virus]] |
| - | [[Category: | + | [[Category: Viral capsid protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:01:07 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:01, 4 May 2008
Hepatitis B Capsid Protein with an N-terminal extension modelled into 8.9 A data.
Overview
Hepatitis B core (HBc) particles have been extensively exploited as carriers for foreign immunological epitopes in the development of multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc particle were grown in PEG 20,000, ammonium sulfate and various types of alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance crystal growth. A crystal grown using MPD as a cryoprotectant diffracted X-rays to 7.7 A resolution and data were collected to 99.6% completeness at 8.9 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 352.3, b = 465.5, c = 645.0 A. The electron-density map reveals a protrusion that is consistent with the N-terminus extending out from the surface of the capsid. The structure presented here supports the idea that N-terminal insertions can be exploited in the development of diagnostic reagents, multicomponent vaccines and delivery vehicles into mammalian cells.
About this Structure
2QIJ is a Single protein structure of sequence from Hepatitis b virus subtype adyw. Full crystallographic information is available from OCA.
Reference
Crystallization and X-ray analysis of the T = 4 particle of hepatitis B capsid protein with an N-terminal extension., Tan WS, McNae IW, Ho KL, Walkinshaw MD, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt, 8):642-7. Epub 2007 Jul 21. PMID:17671358 Page seeded by OCA on Sun May 4 15:01:07 2008
