2qij
From Proteopedia
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'''Hepatitis B Capsid Protein with an N-terminal extension modelled into 8.9 A data.''' | '''Hepatitis B Capsid Protein with an N-terminal extension modelled into 8.9 A data.''' | ||
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[[Category: Tan, W S]] | [[Category: Tan, W S]] | ||
[[Category: Walkinshaw, M D.]] | [[Category: Walkinshaw, M D.]] | ||
| - | [[Category: | + | [[Category: Icosahedral virus]] |
| - | [[Category: | + | [[Category: Viral capsid protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:01:07 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:01, 4 May 2008
Hepatitis B Capsid Protein with an N-terminal extension modelled into 8.9 A data.
Overview
Hepatitis B core (HBc) particles have been extensively exploited as carriers for foreign immunological epitopes in the development of multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc particle were grown in PEG 20,000, ammonium sulfate and various types of alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance crystal growth. A crystal grown using MPD as a cryoprotectant diffracted X-rays to 7.7 A resolution and data were collected to 99.6% completeness at 8.9 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 352.3, b = 465.5, c = 645.0 A. The electron-density map reveals a protrusion that is consistent with the N-terminus extending out from the surface of the capsid. The structure presented here supports the idea that N-terminal insertions can be exploited in the development of diagnostic reagents, multicomponent vaccines and delivery vehicles into mammalian cells.
About this Structure
2QIJ is a Single protein structure of sequence from Hepatitis b virus subtype adyw. Full crystallographic information is available from OCA.
Reference
Crystallization and X-ray analysis of the T = 4 particle of hepatitis B capsid protein with an N-terminal extension., Tan WS, McNae IW, Ho KL, Walkinshaw MD, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt, 8):642-7. Epub 2007 Jul 21. PMID:17671358 Page seeded by OCA on Sun May 4 15:01:07 2008
