2qjz
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2qjz.gif|left|200px]] | [[Image:2qjz.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2qjz", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2qjz| PDB=2qjz | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1''' | '''Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1''' | ||
| Line 28: | Line 25: | ||
[[Category: Vale, R D.]] | [[Category: Vale, R D.]] | ||
[[Category: +tip]] | [[Category: +tip]] | ||
| - | [[Category: | + | [[Category: Calponin homology domain]] |
| - | [[Category: | + | [[Category: Eb1]] |
| - | [[Category: | + | [[Category: Microtubule plus end]] |
| - | [[Category: | + | [[Category: Protein binding]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:05:26 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:05, 4 May 2008
Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1
Overview
Microtubule plus end binding proteins (+TIPs) localize to the dynamic plus ends of microtubules, where they stimulate microtubule growth and recruit signaling molecules. Three main +TIP classes have been identified (XMAP215, EB1, and CLIP-170), but whether they act upon microtubule plus ends through a similar mechanism has not been resolved. Here, we report crystal structures of the tubulin binding domains of XMAP215 (yeast Stu2p and Drosophila Msps), EB1 (yeast Bim1p and human EB1), and CLIP-170 (human), which reveal diverse tubulin binding interfaces. Functional studies, however, reveal a common property that native or artificial dimerization of tubulin binding domains (including chemically induced heterodimers of EB1 and CLIP-170) induces tubulin nucleation/assembly in vitro and, in most cases, plus end tracking in living cells. We propose that +TIPs, although diverse in structure, share a common property of multimerizing tubulin, thus acting as polymerization chaperones that aid in subunit addition to the microtubule plus end.
About this Structure
2QJZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1., Slep KC, Vale RD, Mol Cell. 2007 Sep 21;27(6):976-91. PMID:17889670 Page seeded by OCA on Sun May 4 15:05:26 2008
