2qsc
From Proteopedia
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'''Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide''' | '''Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide''' | ||
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[[Category: Stanfield, R L.]] | [[Category: Stanfield, R L.]] | ||
[[Category: Wilson, I A.]] | [[Category: Wilson, I A.]] | ||
| - | [[Category: | + | [[Category: Aid]] |
| - | [[Category: | + | [[Category: Apoptosis]] |
| - | [[Category: | + | [[Category: Envelope protein]] |
| - | [[Category: | + | [[Category: Fab-peptide complex]] |
| - | [[Category: | + | [[Category: Fusion protein]] |
| - | [[Category: | + | [[Category: Glycoprotein]] |
| - | [[Category: | + | [[Category: Gp120]] |
| - | [[Category: | + | [[Category: Hiv-1]] |
| - | [[Category: | + | [[Category: Host-virus interaction]] |
| - | [[Category: | + | [[Category: Immune system]] |
| - | [[Category: | + | [[Category: Immunoglobulin fold]] |
| - | [[Category: | + | [[Category: Membrane]] |
| - | [[Category: | + | [[Category: Transmembrane]] |
| - | [[Category: | + | [[Category: V3 loop]] |
| - | [[Category: | + | [[Category: Viral immunoevasion]] |
| - | [[Category: | + | [[Category: Virion]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:34:23 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:34, 4 May 2008
Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide
Overview
F425-B4e8 (B4e8) is a monoclonal antibody isolated from a human immunodeficiency virus type 1 (HIV-1)-infected individual that recognizes the V3 variable loop on the gp120 subunit of the viral envelope spike. B4e8 neutralizes a subset of HIV-1 primary isolates from subtypes B, C and D, which places this antibody among the very few human anti-V3 antibodies with notable cross-neutralizing activity. Here, the crystal structure of the B4e8 Fab' fragment in complex with a 24-mer V3 peptide (RP142) at 2.8 A resolution is described. The complex structure reveals that the antibody recognizes a novel V3 loop conformation, featuring a five-residue alpha-turn around the conserved GPGRA apex of the beta-hairpin loop. In agreement with previous mutagenesis analyses, the Fab' interacts primarily with V3 through side-chain contacts with just two residues, Ile(P309) and Arg(P315), while the remaining contacts are to the main chain. The structure helps explain how B4e8 can tolerate a certain degree of sequence variation within V3 and, hence, is able to neutralize an appreciable number of different HIV-1 isolates.
About this Structure
2QSC is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of antibody F425-B4e8 in complex with a V3 peptide reveals a new binding mode for HIV-1 neutralization., Bell CH, Pantophlet R, Schiefner A, Cavacini LA, Stanfield RL, Burton DR, Wilson IA, J Mol Biol. 2008 Jan 25;375(4):969-78. Epub 2007 Nov 13. PMID:18068724 Page seeded by OCA on Sun May 4 15:34:23 2008
Categories: Homo sapiens | Protein complex | Bell, C H. | Schiefner, A. | Stanfield, R L. | Wilson, I A. | Aid | Apoptosis | Envelope protein | Fab-peptide complex | Fusion protein | Glycoprotein | Gp120 | Hiv-1 | Host-virus interaction | Immune system | Immunoglobulin fold | Membrane | Transmembrane | V3 loop | Viral immunoevasion | Virion
