2qvw

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[[Image:2qvw.jpg|left|200px]]
[[Image:2qvw.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2qvw |SIZE=350|CAPTION= <scene name='initialview01'>2qvw</scene>, resolution 3.000&Aring;
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The line below this paragraph, containing "STRUCTURE_2qvw", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] </span>
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|GENE=
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{{STRUCTURE_2qvw| PDB=2qvw | SCENE= }}
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|RELATEDENTRY=[[2ffl|2FFL]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvw OCA], [http://www.ebi.ac.uk/pdbsum/2qvw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qvw RCSB]</span>
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}}
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'''Structure of Giardia Dicer refined against twinned data'''
'''Structure of Giardia Dicer refined against twinned data'''
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[[Category: Doudna, J A.]]
[[Category: Doudna, J A.]]
[[Category: MacRae, I J]]
[[Category: MacRae, I J]]
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[[Category: dicer]]
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[[Category: Dicer]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: rnai,pseudo-merohedral twin]]
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[[Category: Rnai,pseudo-merohedral twin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:47:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:53:56 2008''
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Revision as of 12:47, 4 May 2008

Image:2qvw.jpg

Template:STRUCTURE 2qvw

Structure of Giardia Dicer refined against twinned data


Overview

The crystal structure of the enzyme Dicer from Giardia intestinalis was solved to 3.3 A resolution by MAD using crystals belonging to space group P2(1)2(1)2 [Macrae et al. (2006), Science, 311, 195-198]. These crystals were derived from crystals that diffracted X-rays to 3.0 A resolution but were refractory to structure determination because they were twinned. It is shown here that the original Dicer crystals represent an unusual case of perfect pseudo-merohedral twinning of orthorhombic crystals. Before the twinning problem was overcome, it was possible to calculate a low-resolution electron-density map in space group P4(1) that was used to build a partial molecular model. Experimental phases were sufficient to identify heavy-atom sites that indicated space-group inconsistency, leading to identification of the true space group. This information guided the search for different crystallization conditions that yielded untwinned crystals and ultimately a fully interpretable electron-density map.

About this Structure

2QVW is a Single protein structure of sequence from Giardia intestinalis. Full crystallographic information is available from OCA.

Reference

An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer., MacRae IJ, Doudna JA, Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:17704568 Page seeded by OCA on Sun May 4 15:47:02 2008

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