2qxi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2qxi.jpg|left|200px]]
[[Image:2qxi.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2qxi |SIZE=350|CAPTION= <scene name='initialview01'>2qxi</scene>, resolution 1.000&Aring;
+
The line below this paragraph, containing "STRUCTURE_2qxi", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=K7J:N-(3-CARBOXYPROPANOYL)-L-ALANYL-L-ALANYL-N-[(1S,2R)-1-BENZYL-2-HYDROXYPROPYL]-L-PROLINAMIDE'>K7J</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Stratum_corneum_chymotryptic_enzyme Stratum corneum chymotryptic enzyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.117 3.4.21.117] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= KLK7, PRSS6, SCCE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2qxi| PDB=2qxi | SCENE= }}
-
|RELATEDENTRY=[[2qxg|2QXG]], [[2qxh|2QXH]], [[2qxj|2QXJ]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qxi OCA], [http://www.ebi.ac.uk/pdbsum/2qxi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qxi RCSB]</span>
+
-
}}
+
'''High resolution structure of Human Kallikrein 7 in Complex with Suc-Ala-Ala-Pro-Phe-chloromethylketone'''
'''High resolution structure of Human Kallikrein 7 in Complex with Suc-Ala-Ala-Pro-Phe-chloromethylketone'''
Line 32: Line 29:
[[Category: Magdolen, V.]]
[[Category: Magdolen, V.]]
[[Category: Schechter, N M.]]
[[Category: Schechter, N M.]]
-
[[Category: alternate conformation]]
+
[[Category: Alternate conformation]]
-
[[Category: alternative splicing]]
+
[[Category: Alternative splicing]]
-
[[Category: chloromethyl ketone]]
+
[[Category: Chloromethyl ketone]]
-
[[Category: glycoprotein]]
+
[[Category: Glycoprotein]]
-
[[Category: hydrolase]]
+
[[Category: Hydrolase]]
-
[[Category: protease]]
+
[[Category: Protease]]
-
[[Category: s1 pocket]]
+
[[Category: S1 pocket]]
-
[[Category: secreted]]
+
[[Category: Secreted]]
-
[[Category: serine protease]]
+
[[Category: Serine protease]]
-
[[Category: zymogen]]
+
[[Category: Zymogen]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:52:15 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:54:29 2008''
+

Revision as of 12:52, 4 May 2008

Image:2qxi.jpg

Template:STRUCTURE 2qxi

High resolution structure of Human Kallikrein 7 in Complex with Suc-Ala-Ala-Pro-Phe-chloromethylketone


Overview

hK7 or human stratum corneum chymotryptic enzyme belongs to the human tissue kallikrein (hKs) serine proteinase family and is strongly expressed in the upper layers of the epidermis. It participates in skin desquamation but is also implicated in diverse skin diseases and is a potential biomarker of ovarian cancer. We have solved x-ray structures of recombinant active hK7 at medium and atomic resolution in the presence of the inhibitors succinyl-Ala-Ala-Pro-Phe-chloromethyl ketone and Ala-Ala-Phe-chloromethyl ketone. The most distinguishing features of hK7 are the short 70-80 loop and the unique S1 pocket, which prefers P1 Tyr residues, as shown by kinetic data. Similar to several other kallikreins, the enzyme activity is inhibited by Zn(2+) and Cu(2+) at low micromolar concentrations. Biochemical analyses of the mutants H99A and H41F confirm that only the metal-binding site at His(99) close to the catalytic triad accounts for the noncompetitive Zn(2+) inhibition type. Additionally, hK7 exhibits large positively charged surface patches, representing putative exosites for prime side substrate recognition.

About this Structure

2QXI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Chymotryptic specificity determinants in the 1.0 A structure of the zinc-inhibited human tissue kallikrein 7., Debela M, Hess P, Magdolen V, Schechter NM, Steiner T, Huber R, Bode W, Goettig P, Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16086-91. Epub 2007 Oct 1. PMID:17909180 Page seeded by OCA on Sun May 4 15:52:15 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2qxi"
Personal tools