2qzv
From Proteopedia
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| - | + | {{STRUCTURE_2qzv| PDB=2qzv | SCENE= }} | |
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'''Draft Crystal Structure of the Vault Shell at 9 Angstroms Resolution''' | '''Draft Crystal Structure of the Vault Shell at 9 Angstroms Resolution''' | ||
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[[Category: Rome, L H.]] | [[Category: Rome, L H.]] | ||
[[Category: Sievers, S A.]] | [[Category: Sievers, S A.]] | ||
| - | [[Category: | + | [[Category: Barrel self-assembled from 96 stave]] |
| - | [[Category: | + | [[Category: Cytoplasm]] |
| - | [[Category: | + | [[Category: Innate immunity]] |
| - | [[Category: | + | [[Category: Major vault protein]] |
| - | [[Category: | + | [[Category: Nanocapsule]] |
| - | [[Category: | + | [[Category: Ribonucleoprotein]] |
| - | [[Category: | + | [[Category: Structural protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:59:19 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:59, 4 May 2008
Draft Crystal Structure of the Vault Shell at 9 Angstroms Resolution
Overview
The vault is a highly conserved ribonucleoprotein particle found in all higher eukaryotes. It has a barrel-shaped structure and is composed of the major vault protein (MVP); vault poly(ADP-ribose) polymerase (VPARP); telomerase-associated protein 1 (TEP1); and small untranslated RNA (vRNA). Although its strong conservation and high abundance indicate an important cellular role, the function of the vault is unknown. In humans, vaults have been implicated in multidrug resistance during chemotherapy. Recently, assembly of recombinant vaults has been established in insect cells expressing only MVP. Here, we demonstrate that co-expression of MVP with one or both of the other two vault proteins results in their co-assembly into regularly shaped vaults. Particles assembled from MVP with N-terminal peptide tags of various length are compared. Cryoelectron microscopy (cryoEM) and single-particle image reconstruction methods were used to determine the structure of nine recombinant vaults of various composition, as well as wild-type and TEP1-deficient mouse vaults. Recombinant vaults with MVP N-terminal peptide tags showed internal density that varied in size with the length of the tag. Reconstruction of a recombinant vault with a cysteine-rich tag revealed 48-fold rotational symmetry for the vault. A model is proposed for the organization of MVP within the vault with all of the MVP N termini interacting non-covalently at the vault midsection and 48 copies of MVP forming each half vault. CryoEM difference mapping localized VPARP to three density bands lining the inner surface of the vault. Difference maps designed to localize TEP1 showed only weak density inside of the caps, suggesting that TEP1 may interact with MVP via a small interaction region. In the absence of atomic-resolution structures for either VPARP or TEP1, fold recognition methods were applied. A total of 21 repeats were predicted for the TEP1 WD-repeat domain, suggesting an unusually large beta-propeller fold.
About this Structure
2QZV is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Cryoelectron microscopy imaging of recombinant and tissue derived vaults: localization of the MVP N termini and VPARP., Mikyas Y, Makabi M, Raval-Fernandes S, Harrington L, Kickhoefer VA, Rome LH, Stewart PL, J Mol Biol. 2004 Nov 12;344(1):91-105. PMID:15504404 Page seeded by OCA on Sun May 4 15:59:19 2008
