2r7z

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[[Image:2r7z.gif|left|200px]]
[[Image:2r7z.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2r7z |SIZE=350|CAPTION= <scene name='initialview01'>2r7z</scene>, resolution 3.80&Aring;
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The line below this paragraph, containing "STRUCTURE_2r7z", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=CPT:CIS-PLATINUM-(NH3)2'>CPT</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2r7z| PDB=2r7z | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r7z OCA], [http://www.ebi.ac.uk/pdbsum/2r7z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2r7z RCSB]</span>
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}}
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'''Cisplatin lesion containing RNA polymerase II elongation complex'''
'''Cisplatin lesion containing RNA polymerase II elongation complex'''
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[[Category: Cramer, P.]]
[[Category: Cramer, P.]]
[[Category: Damsma, G E.]]
[[Category: Damsma, G E.]]
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[[Category: arrest]]
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[[Category: Arrest]]
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[[Category: cisplatin lesion]]
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[[Category: Cisplatin lesion]]
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[[Category: damage recognition]]
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[[Category: Damage recognition]]
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[[Category: dna damage]]
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[[Category: Dna damage]]
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[[Category: dna lesion]]
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[[Category: Dna lesion]]
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[[Category: dna-binding]]
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[[Category: Dna-binding]]
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[[Category: elongation complex]]
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[[Category: Elongation complex]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: misincorporation]]
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[[Category: Misincorporation]]
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[[Category: nuclear protein]]
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[[Category: Nuclear protein]]
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[[Category: phosphorylation]]
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[[Category: Phosphorylation]]
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[[Category: rna polymerase ii]]
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[[Category: Rna polymerase ii]]
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[[Category: stalling]]
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[[Category: Stalling]]
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[[Category: tcr]]
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[[Category: Tcr]]
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[[Category: transcription]]
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[[Category: Transcription]]
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[[Category: transcription bubble]]
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[[Category: Transcription bubble]]
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[[Category: transcription- coupled repair]]
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[[Category: Transcription- coupled repair]]
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[[Category: transferase]]
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[[Category: Transferase]]
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[[Category: transferase/dna/rna]]
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[[Category: Transferase/dna/rna]]
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[[Category: transferase/dna/rna complex]]
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[[Category: Transferase/dna/rna complex]]
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[[Category: zinc-finger]]
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[[Category: Zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:24:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:57:27 2008''
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Revision as of 13:24, 4 May 2008

Image:2r7z.gif

Template:STRUCTURE 2r7z

Cisplatin lesion containing RNA polymerase II elongation complex


Overview

The anticancer drug cisplatin forms 1,2-d(GpG) DNA intrastrand cross-links (cisplatin lesions) that stall RNA polymerase II (Pol II) and trigger transcription-coupled DNA repair. Here we present a structure-function analysis of Pol II stalling at a cisplatin lesion in the DNA template. Pol II stalling results from a translocation barrier that prevents delivery of the lesion to the active site. AMP misincorporation occurs at the barrier and also at an abasic site, suggesting that it arises from nontemplated synthesis according to an 'A-rule' known for DNA polymerases. Pol II can bypass a cisplatin lesion that is artificially placed beyond the translocation barrier, even in the presence of a G.A mismatch. Thus, the barrier prevents transcriptional mutagenesis. The stalling mechanism differs from that of Pol II stalling at a photolesion, which involves delivery of the lesion to the active site and lesion-templated misincorporation that blocks transcription.

About this Structure

2R7Z is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Mechanism of transcriptional stalling at cisplatin-damaged DNA., Damsma GE, Alt A, Brueckner F, Carell T, Cramer P, Nat Struct Mol Biol. 2007 Dec;14(12):1127-33. Epub 2007 Nov 11. PMID:17994106 Page seeded by OCA on Sun May 4 16:24:13 2008

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OCA

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