2r8b
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2r8b.jpg|left|200px]] | [[Image:2r8b.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2r8b", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2r8b| PDB=2r8b | SCENE= }} | |
| - | | | + | |
| - | | | + | |
| - | }} | + | |
'''The crystal structure of the protein Atu2452 of unknown function from Agrobacterium tumefaciens str. C58''' | '''The crystal structure of the protein Atu2452 of unknown function from Agrobacterium tumefaciens str. C58''' | ||
| Line 32: | Line 29: | ||
[[Category: Xu, X.]] | [[Category: Xu, X.]] | ||
[[Category: Zheng, H.]] | [[Category: Zheng, H.]] | ||
| - | [[Category: | + | [[Category: Agrobacterium tumefaciens str. c58]] |
| - | [[Category: | + | [[Category: Apc6088]] |
| - | [[Category: | + | [[Category: Mcsg]] |
| - | [[Category: | + | [[Category: Midwest center for structural genomic]] |
| - | [[Category: | + | [[Category: Protein atu2452]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi-2]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Unknown function]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:25:00 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:25, 4 May 2008
The crystal structure of the protein Atu2452 of unknown function from Agrobacterium tumefaciens str. C58
Overview
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
About this Structure
2R8B is a Single protein structure of sequence from Agrobacterium tumefaciens str. c58. Full crystallographic information is available from OCA.
Reference
In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461 Page seeded by OCA on Sun May 4 16:25:00 2008
Categories: Agrobacterium tumefaciens str. c58 | Single protein | Edwards, A M. | Joachimiak, A. | MCSG, Midwest Center for Structural Genomics. | Savchenko, A. | Tan, K. | Xu, X. | Zheng, H. | Apc6088 | Mcsg | Midwest center for structural genomic | Protein atu2452 | Protein structure initiative | Psi-2 | Structural genomic | Unknown function
