2r8w

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[[Image:2r8w.gif|left|200px]]
[[Image:2r8w.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2r8w |SIZE=350|CAPTION= <scene name='initialview01'>2r8w</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_2r8w", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|GENE= AGR_C_1641 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176299 Agrobacterium tumefaciens str. C58])
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0329 DapA], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00408 DHDPS-like]</span>
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{{STRUCTURE_2r8w| PDB=2r8w | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r8w OCA], [http://www.ebi.ac.uk/pdbsum/2r8w PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2r8w RCSB]</span>
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}}
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'''The crystal structure of dihydrodipicolinate synthase (Atu0899) from Agrobacterium tumefaciens str. C58'''
'''The crystal structure of dihydrodipicolinate synthase (Atu0899) from Agrobacterium tumefaciens str. C58'''
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[[Category: Xu, X.]]
[[Category: Xu, X.]]
[[Category: Zheng, H.]]
[[Category: Zheng, H.]]
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[[Category: agrobacterium tumefaciens str. c58]]
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[[Category: Agrobacterium tumefaciens str. c58]]
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[[Category: apc7498]]
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[[Category: Apc7498]]
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[[Category: dihydrodipicolinate synthase]]
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[[Category: Dihydrodipicolinate synthase]]
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[[Category: lyase]]
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[[Category: Lyase]]
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[[Category: mcsg]]
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[[Category: Mcsg]]
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[[Category: midwest center for structural genomic]]
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[[Category: Midwest center for structural genomic]]
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[[Category: protein structure initiative]]
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[[Category: Protein structure initiative]]
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[[Category: psi-2]]
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[[Category: Psi-2]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:26:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:57:41 2008''
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Revision as of 13:26, 4 May 2008

Image:2r8w.gif

Template:STRUCTURE 2r8w

The crystal structure of dihydrodipicolinate synthase (Atu0899) from Agrobacterium tumefaciens str. C58


Overview

We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.

About this Structure

2R8W is a Single protein structure of sequence from Agrobacterium tumefaciens str. c58. Full crystallographic information is available from OCA.

Reference

In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461 Page seeded by OCA on Sun May 4 16:26:16 2008

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