2r9z

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[[Image:2r9z.jpg|left|200px]]
[[Image:2r9z.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2r9z |SIZE=350|CAPTION= <scene name='initialview01'>2r9z</scene>, resolution 2.10&Aring;
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The line below this paragraph, containing "STRUCTURE_2r9z", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Residue+A+464'>AC1</scene>, <scene name='pdbsite=AC2:Ni+Binding+Site+For+Residue+A+466'>AC2</scene>, <scene name='pdbsite=AC3:Cl+Binding+Site+For+Residue+B+464'>AC3</scene>, <scene name='pdbsite=AC4:Ni+Binding+Site+For+Residue+B+466'>AC4</scene>, <scene name='pdbsite=AC5:Ni+Binding+Site+For+Residue+A+468'>AC5</scene>, <scene name='pdbsite=AC6:Ni+Binding+Site+For+Residue+B+468'>AC6</scene>, <scene name='pdbsite=AC7:Fad+Binding+Site+For+Residue+A+500'>AC7</scene> and <scene name='pdbsite=AC8:Fad+Binding+Site+For+Residue+B+500'>AC8</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2r9z| PDB=2r9z | SCENE= }}
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|RELATEDENTRY=[[2rab|2RAB]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r9z OCA], [http://www.ebi.ac.uk/pdbsum/2r9z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2r9z RCSB]</span>
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}}
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'''Glutathione amide reductase from Chromatium gracile'''
'''Glutathione amide reductase from Chromatium gracile'''
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[[Category: Vergauwen, B.]]
[[Category: Vergauwen, B.]]
[[Category: Vos, D De.]]
[[Category: Vos, D De.]]
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[[Category: fad]]
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[[Category: Fad]]
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[[Category: glutathione]]
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[[Category: Glutathione]]
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[[Category: gr]]
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[[Category: Gr]]
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[[Category: nad]]
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[[Category: Nad]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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[[Category: substrate specificity]]
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[[Category: Substrate specificity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:30:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:57:59 2008''
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Revision as of 13:30, 4 May 2008

Image:2r9z.jpg

Template:STRUCTURE 2r9z

Glutathione amide reductase from Chromatium gracile


Overview

Glutathione reductase (GR) plays a vital role in maintaining the antioxidant levels of the cytoplasm by catalyzing the reduction of glutathione disulfide to reduced glutathione, thereby using NADPH and flavin adenine dinucleotide as cofactors. Chromatiaceae have evolved an unusual homolog that prefers both a modified substrate (glutathione amide disulfide [GASSAG]) and a different cofactor (NADH). Herein, we present the crystal structure of the Chromatium gracile glutathione amide reductase (GAR) both alone and in complex with NAD(+). An altered charge distribution in the GASSAG binding pocket explains the difference in substrate specificity. The NADH binding pocket of GAR differs from that of wild-type GR as well as that of a low active GR that was engineered to mimic NADH binding. Based on the GAR structure, we propose two attractive rationales for producing an efficient GR enzyme with NADH specificity.

About this Structure

2R9Z is a Single protein structure of sequence from Marichromatium gracile. Full crystallographic information is available from OCA.

Reference

Understanding nicotinamide dinucleotide cofactor and substrate specificity in class I flavoprotein disulfide oxidoreductases: crystallographic analysis of a glutathione amide reductase., Van Petegem F, De Vos D, Savvides S, Vergauwen B, Van Beeumen J, J Mol Biol. 2007 Dec 7;374(4):883-9. Epub 2007 Sep 29. PMID:17977556 Page seeded by OCA on Sun May 4 16:30:32 2008

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OCA

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