2raw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2raw.gif|left|200px]]
[[Image:2raw.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2raw |SIZE=350|CAPTION= <scene name='initialview01'>2raw</scene>, resolution 2.40&Aring;
+
The line below this paragraph, containing "STRUCTURE_2raw", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= BIRC5, API4, IAP4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), CDCA8, PESCRG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2raw| PDB=2raw | SCENE= }}
-
|RELATEDENTRY=[[2rax|2RAX]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2raw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2raw OCA], [http://www.ebi.ac.uk/pdbsum/2raw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2raw RCSB]</span>
+
-
}}
+
'''Crystal structure of the Borealin-Survivin complex'''
'''Crystal structure of the Borealin-Survivin complex'''
Line 26: Line 23:
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Hymowitz, S G.]]
[[Category: Hymowitz, S G.]]
-
[[Category: alternative splicing]]
+
[[Category: Alternative splicing]]
-
[[Category: apoptosis]]
+
[[Category: Apoptosis]]
-
[[Category: bir]]
+
[[Category: Bir]]
-
[[Category: cell cycle]]
+
[[Category: Cell cycle]]
-
[[Category: cell division]]
+
[[Category: Cell division]]
-
[[Category: centromere]]
+
[[Category: Centromere]]
-
[[Category: chromosomal passender complex]]
+
[[Category: Chromosomal passender complex]]
-
[[Category: chromosomal protein]]
+
[[Category: Chromosomal protein]]
-
[[Category: cytoplasm]]
+
[[Category: Cytoplasm]]
-
[[Category: dasrab]]
+
[[Category: Dasrab]]
-
[[Category: iap]]
+
[[Category: Iap]]
-
[[Category: metal-binding]]
+
[[Category: Metal-binding]]
-
[[Category: mitosis]]
+
[[Category: Mitosis]]
-
[[Category: nucleus]]
+
[[Category: Nucleus]]
-
[[Category: phosphorylation]]
+
[[Category: Phosphorylation]]
-
[[Category: polymorphism]]
+
[[Category: Polymorphism]]
-
[[Category: protease inhibitor]]
+
[[Category: Protease inhibitor]]
-
[[Category: thiol protease inhibitor]]
+
[[Category: Thiol protease inhibitor]]
-
[[Category: zinc]]
+
[[Category: Zinc]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:34:10 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:58:19 2008''
+

Revision as of 13:34, 4 May 2008

Image:2raw.gif

Template:STRUCTURE 2raw

Crystal structure of the Borealin-Survivin complex


Overview

Survivin is a member of the IAP (inhibitor of apoptosis) protein family, defined in part by the presence of a zinc-binding baculoviral inhibitory repeat (BIR) domain. Most BIR domains bind short sequences beginning with alanine, and in this manner, they recognize and block the action of key targets in apoptotic pathways. However, Survivin binds only very weakly to typical IAP ligands. Unique features of Survivin are the long C-terminal helix following the BIR domain and a short segment (linking the helix and BIR domains) that mediates Survivin homodimerization. Despite this detailed knowledge of the structure of Survivin itself, there is a current lack of understanding about how Survivin recognizes cellular binding partners, and consequently, many questions about Survivin function remain unanswered. We determined two co-crystal structures of Survivin and a minimal binding fragment from the chromosomal passenger protein Borealin, a well validated functional interactor. The interaction between Survivin and Borealin involves extensive packing between the long C-terminal helix of Survivin and a long Borealin helix. Surprisingly, an additional important interaction occurs between the Survivin homodimerization interface and a short segment of Borealin. This segment both structurally mimics and displaces one Survivin monomer. The relevance of this unexpected interaction was tested by mutagenesis of two key Borealin residues. Mutant Borealin introduced into HeLa cells failed to localize properly during mitosis and also caused mislocalization of other chromosomal passenger proteins. This suggests that the mutant is dominant-negative and confirms the functional importance of the interaction surface identified in the crystal structures.

About this Structure

2RAW is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The mitotic regulator Survivin binds as a monomer to its functional interactor Borealin., Bourhis E, Hymowitz SG, Cochran AG, J Biol Chem. 2007 Nov 30;282(48):35018-23. Epub 2007 Sep 19. PMID:17881355 Page seeded by OCA on Sun May 4 16:34:10 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2raw"
Personal tools