2rfm

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[[Image:2rfm.jpg|left|200px]]
[[Image:2rfm.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2rfm |SIZE=350|CAPTION= <scene name='initialview01'>2rfm</scene>, resolution 1.650&Aring;
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The line below this paragraph, containing "STRUCTURE_2rfm", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+190'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+B+190'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+B+191'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+B+192'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+A+191'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+A+193'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Residue+B+193'>AC7</scene>, <scene name='pdbsite=AC8:Cl+Binding+Site+For+Residue+B+194'>AC8</scene>, <scene name='pdbsite=AC9:Cl+Binding+Site+For+Residue+B+195'>AC9</scene>, <scene name='pdbsite=BC1:144+Binding+Site+For+Residue+A+194'>BC1</scene>, <scene name='pdbsite=BC2:Bu2+Binding+Site+For+Residue+B+197'>BC2</scene>, <scene name='pdbsite=BC3:Bu2+Binding+Site+For+Residue+B+198'>BC3</scene>, <scene name='pdbsite=BC4:Bu2+Binding+Site+For+Residue+A+195'>BC4</scene>, <scene name='pdbsite=BC5:Bu2+Binding+Site+For+Residue+A+196'>BC5</scene>, <scene name='pdbsite=BC6:Gol+Binding+Site+For+Residue+B+199'>BC6</scene>, <scene name='pdbsite=BC7:Gol+Binding+Site+For+Residue+B+200'>BC7</scene>, <scene name='pdbsite=BC8:Gol+Binding+Site+For+Residue+A+197'>BC8</scene>, <scene name='pdbsite=BC9:Gol+Binding+Site+For+Residue+B+201'>BC9</scene>, <scene name='pdbsite=CC1:Gol+Binding+Site+For+Residue+A+198'>CC1</scene> and <scene name='pdbsite=CC2:Gol+Binding+Site+For+Residue+A+199'>CC2</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=144:TRIS-HYDROXYMETHYL-METHYL-AMMONIUM'>144</scene>, <scene name='pdbligand=BU2:1,3-BUTANEDIOL'>BU2</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= TVG1472127 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=50339 Thermoplasma volcanium])
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-->
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|DOMAIN=
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{{STRUCTURE_2rfm| PDB=2rfm | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rfm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rfm OCA], [http://www.ebi.ac.uk/pdbsum/2rfm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2rfm RCSB]</span>
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}}
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'''Structure of a Thermophilic Ankyrin Repeat Protein'''
'''Structure of a Thermophilic Ankyrin Repeat Protein'''
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[[Category: Stubbs, M T.]]
[[Category: Stubbs, M T.]]
[[Category: Weininger, U.]]
[[Category: Weininger, U.]]
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[[Category: ank repeat]]
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[[Category: Ank repeat]]
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[[Category: ankyrin repeat]]
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[[Category: Ankyrin repeat]]
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[[Category: protein binding]]
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[[Category: Protein binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:49:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:59:52 2008''
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Revision as of 13:49, 4 May 2008

Image:2rfm.jpg

Template:STRUCTURE 2rfm

Structure of a Thermophilic Ankyrin Repeat Protein


Overview

Repeat proteins are widespread in nature, with many of them functioning as binding molecules in protein-protein recognition. Their simple structural architecture is used in biotechnology for generating proteins with high affinities to target proteins. Recent folding studies of ankyrin repeat (AR) proteins revealed a new mechanism of protein folding. The formation of an intermediate state is rate limiting in the folding reaction, suggesting a scaffold function of this transient state for intrinsically less stable ARs. To investigate a possible common mechanism of AR folding, we studied the structure and folding of a new thermophilic AR protein (tANK) identified in the archaeon Thermoplasma volcanium. The x-ray structure of the evolutionary much older tANK revealed high homology to the human CDK inhibitor p19(INK4d), whose sequence was used for homology search. As for p19(INK4d), equilibrium and kinetic folding analyses classify tANK to the family of sequential three-state folding proteins, with an unusual fast equilibrium between native and intermediate state. Under equilibrium conditions, the intermediate can be populated to >90%, allowing characterization on a residue-by-residue level using NMR spectroscopy. These data clearly show that the three C-terminal ARs are natively folded in the intermediate state, whereas native cross-peaks for the rest of the molecule are missing. Therefore, the formation of a stable folding unit consisting of three ARs is the necessary rate-limiting step before AR 1 and 2 can assemble to form the native state.

About this Structure

2RFM is a Single protein structure of sequence from Thermoplasma volcanium. Full crystallographic information is available from OCA.

Reference

Structural insights into an equilibrium folding intermediate of an archaeal ankyrin repeat protein., Low C, Weininger U, Neumann P, Klepsch M, Lilie H, Stubbs MT, Balbach J, Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3779-84. Epub 2008 Feb 27. PMID:18305166 Page seeded by OCA on Sun May 4 16:49:38 2008

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