2shk

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[[Image:2shk.jpg|left|200px]]
[[Image:2shk.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2shk |SIZE=350|CAPTION= <scene name='initialview01'>2shk</scene>, resolution 2.6&Aring;
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The line below this paragraph, containing "STRUCTURE_2shk", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=PIP:A+Walker+Type-A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>PIP</scene>, <scene name='pdbsite=POP:A+Walker+Type-A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>POP</scene>, <scene name='pdbsite=SAS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SAS</scene> and <scene name='pdbsite=SBS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SBS</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= AROL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi])
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-->
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|DOMAIN=
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{{STRUCTURE_2shk| PDB=2shk | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2shk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2shk OCA], [http://www.ebi.ac.uk/pdbsum/2shk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2shk RCSB]</span>
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}}
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'''THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI COMPLEXED WITH ADP'''
'''THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI COMPLEXED WITH ADP'''
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[[Category: Krell, T.]]
[[Category: Krell, T.]]
[[Category: Lapthorn, A J.]]
[[Category: Lapthorn, A J.]]
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[[Category: adp]]
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[[Category: Adp]]
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[[Category: p-loop protein]]
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[[Category: P-loop protein]]
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[[Category: phosphoryl transfer]]
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[[Category: Phosphoryl transfer]]
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[[Category: shikimate kinase]]
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[[Category: Shikimate kinase]]
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[[Category: shikimate pathway]]
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[[Category: Shikimate pathway]]
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[[Category: transferase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:18:57 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:03:00 2008''
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Revision as of 14:18, 4 May 2008

Image:2shk.jpg

Template:STRUCTURE 2shk

THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI COMPLEXED WITH ADP


Overview

Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia coli has been crystallized by the vapour-diffusion method using sodium chloride as a precipitant. Mass spectrometry was used to confirm the purity of the shikimate kinase and dynamic light scattering was used to assess conditions for the monodispersity of the enzyme. The crystals are tetragonal, space group P4(1)2(1)2 or enantiomorph with cell dimensions a = b = 108.5 and c = 92.8 A (at 100 K). Native crystals diffract to better than 2.6 A on a synchrotron X-ray source. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 3.6 A(3) Da(-1).

About this Structure

2SHK is a Single protein structure of sequence from Erwinia chrysanthemi. Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:15299895 Page seeded by OCA on Sun May 4 17:18:57 2008

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