2tbs

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[[Image:2tbs.jpg|left|200px]]
[[Image:2tbs.jpg|left|200px]]
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{{Structure
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|PDB= 2tbs |SIZE=350|CAPTION= <scene name='initialview01'>2tbs</scene>, resolution 1.8&Aring;
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The line below this paragraph, containing "STRUCTURE_2tbs", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span>
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{{STRUCTURE_2tbs| PDB=2tbs | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2tbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2tbs OCA], [http://www.ebi.ac.uk/pdbsum/2tbs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2tbs RCSB]</span>
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'''COLD-ADAPTION OF ENZYMES: STRUCTURAL COMPARISON BETWEEN SALMON AND BOVINE TRYPSINS'''
'''COLD-ADAPTION OF ENZYMES: STRUCTURAL COMPARISON BETWEEN SALMON AND BOVINE TRYPSINS'''
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==About this Structure==
==About this Structure==
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2TBS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmo_salar Salmo salar]. This structure supersedes the now removed PDB entry 1TBS. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TBS OCA].
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2TBS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmo_salar Salmo salar]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tbs 1tbs]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TBS OCA].
==Reference==
==Reference==
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[[Category: Trypsin]]
[[Category: Trypsin]]
[[Category: Smalas, A O.]]
[[Category: Smalas, A O.]]
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[[Category: hydrolase(serine proteinase)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:22:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:03:31 2008''
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Revision as of 14:22, 4 May 2008

Template:STRUCTURE 2tbs

COLD-ADAPTION OF ENZYMES: STRUCTURAL COMPARISON BETWEEN SALMON AND BOVINE TRYPSINS


Overview

The crystal structure of an anionic form of salmon trypsin has been determined at 1.82 A resolution. We report the first structure of a trypsin from a phoikilothermic organism in a detailed comparison to mammalian trypsins in order to look for structural rationalizations for the cold-adaption features of salmon trypsin. This form of salmon trypsin (ST II) comprises 222 residues, and is homologous to bovine trypsin (BT) in about 65% of the primary structure. The tertiary structures are similar, with an overall displacement in main chain atomic positions between salmon trypsin and various crystal structures of bovine trypsin of about 0.8 A. Intramolecular hydrogen bonds and hydrophobic interactions are compared and discussed in order to estimate possible differences in molecular flexibility which might explain the higher catalytic efficiency and lower thermostability of salmon trypsin compared to bovine trypsin. No overall differences in intramolecular interactions are detected between the two structures, but there are differences in certain regions of the structures which may explain some of the observed differences in physical properties. The distribution of charged residues is different in the two trypsins, and the impact this might have on substrate affinity has been discussed.

About this Structure

2TBS is a Single protein structure of sequence from Salmo salar. This structure supersedes the now removed PDB entry 1tbs. Full crystallographic information is available from OCA.

Reference

Cold adaption of enzymes: structural comparison between salmon and bovine trypsins., Smalas AO, Heimstad ES, Hordvik A, Willassen NP, Male R, Proteins. 1994 Oct;20(2):149-66. PMID:7846025 Page seeded by OCA on Sun May 4 17:22:29 2008

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