2uvr

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[[Image:2uvr.jpg|left|200px]]
[[Image:2uvr.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2uvr |SIZE=350|CAPTION= <scene name='initialview01'>2uvr</scene>, resolution 2.90&Aring;
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The line below this paragraph, containing "STRUCTURE_2uvr", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Dgt+Binding+Site+For+Chain+A'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=8OG:8-OXO-2&#39;-DEOXY-GUANOSINE-5&#39;-MONOPHOSPHATE'>8OG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DGT:2&#39;-DEOXYGUANOSINE-5&#39;-TRIPHOSPHATE'>DGT</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2uvr| PDB=2uvr | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvr OCA], [http://www.ebi.ac.uk/pdbsum/2uvr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2uvr RCSB]</span>
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}}
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'''CRYSTAL STRUCTURES OF MUTANT DPO4 DNA POLYMERASES WITH 8-OXOG CONTAINING DNA TEMPLATE-PRIMER CONSTRUCTS'''
'''CRYSTAL STRUCTURES OF MUTANT DPO4 DNA POLYMERASES WITH 8-OXOG CONTAINING DNA TEMPLATE-PRIMER CONSTRUCTS'''
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[[Category: Egli, M.]]
[[Category: Egli, M.]]
[[Category: Irimia, A.]]
[[Category: Irimia, A.]]
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[[Category: 7]]
 
[[Category: 8-dihydro-8-oxodeoxyguanosine]]
[[Category: 8-dihydro-8-oxodeoxyguanosine]]
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[[Category: dna damage]]
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[[Category: Dna damage]]
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[[Category: dna repair]]
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[[Category: Dna repair]]
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[[Category: dna replication]]
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[[Category: Dna replication]]
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[[Category: dna- binding]]
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[[Category: Dna- binding]]
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[[Category: dna-binding]]
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[[Category: Dna-binding]]
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[[Category: dna-directed dna polymerase]]
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[[Category: Dna-directed dna polymerase]]
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[[Category: magnesium]]
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[[Category: Magnesium]]
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[[Category: metal- binding]]
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[[Category: Metal- binding]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: mutator protein]]
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[[Category: Mutator protein]]
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[[Category: nucleotidyltransferase]]
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[[Category: Nucleotidyltransferase]]
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[[Category: p2 dna polymerase iv]]
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[[Category: P2 dna polymerase iv]]
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[[Category: transferase]]
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[[Category: Transferase]]
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[[Category: translesion dna polymerase]]
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[[Category: Translesion dna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:36:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:05:09 2008''
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Revision as of 14:36, 4 May 2008

Image:2uvr.jpg

Template:STRUCTURE 2uvr

CRYSTAL STRUCTURES OF MUTANT DPO4 DNA POLYMERASES WITH 8-OXOG CONTAINING DNA TEMPLATE-PRIMER CONSTRUCTS


Overview

Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) has been shown to catalyze bypass of 7,8-dihydro-8-oxodeoxyguanosine (8-oxoG) in a highly efficient and relatively accurate manner. Crystal structures have revealed a potential role for Arg(332) in stabilizing the anti conformation of the 8-oxoG template base by means of a hydrogen bond or ion-dipole pair, which results in an increased enzymatic efficiency for dCTP insertion and makes formation of a Hoogsteen pair between 8-oxoG and dATP less favorable. Site-directed mutagenesis was used to replace Arg(332) with Ala, Glu, Leu, or His in order to probe the importance of Arg(332) in accurate and efficient bypass of 8-oxoG. The double mutant Ala(331)Ala(332) was also prepared to address the contribution of Arg(331). Transientstate kinetic results suggest that Glu(332) retains fidelity against bypass of 8-oxoG that is similar to wild type Dpo4, a result that was confirmed by tandem mass spectrometric analysis of full-length extension products. A crystal structure of the Dpo4 Glu(332) mutant and 8-oxoG:C pair revealed water-mediated hydrogen bonds between Glu(332) and the O-8 atom of 8-oxoG. The space normally occupied by Arg(332) side chain is empty in the crystal structures of the Ala(332) mutant. Two other crystal structures show that a Hoogsteen base pair is formed between 8-oxoG and A in the active site of both Glu(332) and Ala(332) mutants. These results support the view that a bond between Arg(332) and 8-oxoG plays a role in determining the fidelity and efficiency of Dpo4-catalyzed bypass of the lesion.

About this Structure

2UVR is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

Hydrogen bonding of 7,8-dihydro-8-oxodeoxyguanosine with a charged residue in the little finger domain determines miscoding events in Sulfolobus solfataricus DNA polymerase Dpo4., Eoff RL, Irimia A, Angel KC, Egli M, Guengerich FP, J Biol Chem. 2007 Jul 6;282(27):19831-43. Epub 2007 Apr 27. PMID:17468100 Page seeded by OCA on Sun May 4 17:36:09 2008

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