2uwa

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[[Image:2uwa.gif|left|200px]]
[[Image:2uwa.gif|left|200px]]
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{{Structure
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|PDB= 2uwa |SIZE=350|CAPTION= <scene name='initialview01'>2uwa</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_2uwa", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+C'>AC1</scene>
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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{{STRUCTURE_2uwa| PDB=2uwa | SCENE= }}
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|RELATEDENTRY=[[2uwb|2UWB]], [[2uwc|2UWC]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uwa OCA], [http://www.ebi.ac.uk/pdbsum/2uwa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2uwa RCSB]</span>
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'''CRYSTAL STRUCTURE OF THE NASTURTIUM SEEDLING XYLOGLUCANASE ISOFORM NXG1'''
'''CRYSTAL STRUCTURE OF THE NASTURTIUM SEEDLING XYLOGLUCANASE ISOFORM NXG1'''
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[[Category: Michel, G.]]
[[Category: Michel, G.]]
[[Category: Teeri, T T.]]
[[Category: Teeri, T T.]]
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[[Category: family gh16]]
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[[Category: Family gh16]]
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[[Category: glycosidase]]
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[[Category: Glycosidase]]
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[[Category: glycoside hydrolase]]
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[[Category: Glycoside hydrolase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: tropaeolum majus xyloglucanase]]
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[[Category: Tropaeolum majus xyloglucanase]]
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[[Category: xyloglucan-endo-transferase]]
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[[Category: Xyloglucan-endo-transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:38:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:05:24 2008''
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Revision as of 14:38, 4 May 2008

Image:2uwa.gif

Template:STRUCTURE 2uwa

CRYSTAL STRUCTURE OF THE NASTURTIUM SEEDLING XYLOGLUCANASE ISOFORM NXG1


Overview

High-resolution, three-dimensional structures of the archetypal glycoside hydrolase family 16 (GH16) endo-xyloglucanases Tm-NXG1 and Tm-NXG2 from nasturtium (Tropaeolum majus) have been solved by x-ray crystallography. Key structural features that modulate the relative rates of substrate hydrolysis to transglycosylation in the GH16 xyloglucan-active enzymes were identified by structure-function studies of the recombinantly expressed enzymes in comparison with data for the strict xyloglucan endo-transglycosylase Ptt-XET16-34 from hybrid aspen (Populus tremula x Populus tremuloides). Production of the loop deletion variant Tm-NXG1-DeltaYNIIG yielded an enzyme that was structurally similar to Ptt-XET16-34 and had a greatly increased transglycosylation:hydrolysis ratio. Comprehensive bioinformatic analyses of XTH gene products, together with detailed kinetic data, strongly suggest that xyloglucanase activity has evolved as a gain of function in an ancestral GH16 XET to meet specific biological requirements during seed germination, fruit ripening, and rapid wall expansion.

About this Structure

2UWA is a Single protein structure of sequence from Tropaeolum majus. Full crystallographic information is available from OCA.

Reference

Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: biological implications for cell wall metabolism., Baumann MJ, Eklof JM, Michel G, Kallas AM, Teeri TT, Czjzek M, Brumer H 3rd, Plant Cell. 2007 Jun;19(6):1947-63. Epub 2007 Jun 8. PMID:17557806 Page seeded by OCA on Sun May 4 17:38:51 2008

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