2v46

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[[Image:2v46.gif|left|200px]]
[[Image:2v46.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2v46 |SIZE=350|CAPTION= <scene name='initialview01'>2v46</scene>, resolution 3.50&Aring;
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The line below this paragraph, containing "STRUCTURE_2v46", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=5MU:5-METHYLURIDINE+5&#39;-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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{{STRUCTURE_2v46| PDB=2v46 | SCENE= }}
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|RELATEDENTRY=[[1fjg|1FJG]], [[1gix|1GIX]], [[1i94|1I94]], [[1i95|1I95]], [[1i96|1I96]], [[1i97|1I97]], [[1ibk|1IBK]], [[1ibl|1IBL]], [[1ibm|1IBM]], [[1j5e|1J5E]], [[1jgo|1JGO]], [[1jgp|1JGP]], [[1jgq|1JGQ]], [[1l1u|1L1U]], [[1n32|1N32]], [[1n33|1N33]], [[1n34|1N34]], [[1n36|1N36]], [[1pns|1PNS]], [[1pnx|1PNX]], [[1xmo|1XMO]], [[1xmq|1XMQ]], [[1xnq|1XNQ]], [[1xnr|1XNR]], [[1yl4|1YL4]], [[2b64|2B64]], [[2b9m|2B9M]], [[2b9o|2B9O]], [[2f4v|2F4V]], [[2j00|2J00]], [[2j02|2J02]], [[2uu9|2UU9]], [[2uua|2UUA]], [[2uub|2UUB]], [[2uuc|2UUC]], [[2uxb|2UXB]], [[2uxc|2UXC]], [[2v47|2V47]], [[2v48|2V48]], [[2v49|2V49]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v46 OCA], [http://www.ebi.ac.uk/pdbsum/2v46 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2v46 RCSB]</span>
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}}
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'''STRUCTURE OF THE RIBOSOME RECYCLING FACTOR BOUND TO THE THERMUS THERMOPHILUS 70S RIBOSOME WITH MRNA, ASL-PHE AND TRNA-FMET (PART 1 OF 4). THIS FILE CONTAINS THE 30S SUBUNIT, MRNA, P-SITE ASL, E-SITE TRNA AND RRF FOR MOLECULE 1.'''
'''STRUCTURE OF THE RIBOSOME RECYCLING FACTOR BOUND TO THE THERMUS THERMOPHILUS 70S RIBOSOME WITH MRNA, ASL-PHE AND TRNA-FMET (PART 1 OF 4). THIS FILE CONTAINS THE 30S SUBUNIT, MRNA, P-SITE ASL, E-SITE TRNA AND RRF FOR MOLECULE 1.'''
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[[Category: Selmer, M.]]
[[Category: Selmer, M.]]
[[Category: Weixlbaumer, A.]]
[[Category: Weixlbaumer, A.]]
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[[Category: coiled coil]]
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[[Category: Coiled coil]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: mrna]]
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[[Category: Mrna]]
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[[Category: protein biosynthesis]]
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[[Category: Protein biosynthesis]]
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[[Category: recycling]]
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[[Category: Recycling]]
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[[Category: ribonucleoprotein]]
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[[Category: Ribonucleoprotein]]
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[[Category: ribosomal protein]]
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[[Category: Ribosomal protein]]
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[[Category: ribosome]]
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[[Category: Ribosome]]
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[[Category: rna-binding]]
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[[Category: Rna-binding]]
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[[Category: rrf]]
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[[Category: Rrf]]
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[[Category: rrna-binding]]
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[[Category: Rrna-binding]]
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[[Category: translation]]
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[[Category: Translation]]
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[[Category: trna]]
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[[Category: Trna]]
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[[Category: trna-binding]]
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[[Category: Trna-binding]]
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[[Category: zinc]]
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[[Category: Zinc]]
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[[Category: zinc-finger]]
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[[Category: Zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:11:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:08:18 2008''
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Revision as of 15:11, 4 May 2008

Image:2v46.gif

Template:STRUCTURE 2v46

STRUCTURE OF THE RIBOSOME RECYCLING FACTOR BOUND TO THE THERMUS THERMOPHILUS 70S RIBOSOME WITH MRNA, ASL-PHE AND TRNA-FMET (PART 1 OF 4). THIS FILE CONTAINS THE 30S SUBUNIT, MRNA, P-SITE ASL, E-SITE TRNA AND RRF FOR MOLECULE 1.


Overview

In bacteria, disassembly of the ribosome at the end of translation is facilitated by an essential protein factor termed ribosome recycling factor (RRF), which works in concert with elongation factor G. Here we describe the crystal structure of the Thermus thermophilus RRF bound to a 70S ribosomal complex containing a stop codon in the A site, a transfer RNA anticodon stem-loop in the P site and tRNA(fMet) in the E site. The work demonstrates that structures of translation factors bound to 70S ribosomes can be determined at reasonably high resolution. Contrary to earlier reports, we did not observe any RRF-induced changes in bridges connecting the two subunits. This suggests that such changes are not a direct requirement for or consequence of RRF binding but possibly arise from the subsequent stabilization of a hybrid state of the ribosome.

About this Structure

2V46 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the ribosome recycling factor bound to the ribosome., Weixlbaumer A, Petry S, Dunham CM, Selmer M, Kelley AC, Ramakrishnan V, Nat Struct Mol Biol. 2007 Aug;14(8):733-7. Epub 2007 Jul 29. PMID:17660830 Page seeded by OCA on Sun May 4 18:11:25 2008

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