1a70
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1a70" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a70, resolution 1.7Å" /> '''SPINACH FERREDOXIN'''...)
Next diff →
Revision as of 08:31, 20 November 2007
|
SPINACH FERREDOXIN
Overview
Ferredoxin I (Fd I) from Spinacia oleracea is composed of 97 amino-acid, residues and a [2Fe-2S] cluster. The crystal structure of the E92K mutant, of Fd I was solved by molecular replacement and refined to an R factor of, 19.6% for 11755 reflections at 1.7 A resolution. The overall structure and, the active centre of spinach Fd is highly conserved with respect to, ferredoxins of known structure. The E92K mutation appears to disturb a, hydrogen-bond network which stabilizes the loop bearing the [2Fe-2S], cluster. This observation provides a rationale for the reduced, electron-transfer efficiency displayed by the E92K mutant. Inspection of, the crystal packing reveals that the side chain of Lys92 is engaged in an, intermolecular interaction with Asp26 of a symmetry-related molecule. This, feature may explain why only the mutant E92K and not wild-type Fd I could, be successfully crystallized.
About this Structure
1A70 is a Single protein structure of sequence from Spinacia oleracea with FES as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the mutant E92K of [2Fe-2S] ferredoxin I from Spinacia oleracea at 1.7 A resolution., Binda C, Coda A, Aliverti A, Zanetti G, Mattevi A, Acta Crystallogr D Biol Crystallogr. 1998 Nov 1;54(Pt 6 Pt 2):1353-8. PMID:10089511
Page seeded by OCA on Tue Nov 20 10:39:02 2007
