1a77
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(New page: 200px<br /><applet load="1a77" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a77, resolution 2.00Å" /> '''FLAP ENDONUCLEASE-1 ...)
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Revision as of 08:32, 20 November 2007
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FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII
Overview
Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an, essential enzyme for eukaryotic DNA replication and repair. The crystal, structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 A, resolution, reveals an active site with two metal ions residing on top of, a deep cleft where several conserved acidic residues are clustered. Near, the active site, a long flexible loop comprised of many basic and aromatic, residues forms a hole large enough to accommodate the DNA substrate., Deletion mutations in this loop significantly decreased the nuclease, activity and specificity of FEN-1, suggesting that the loop is critical, for recognition and cleavage of the junction between single and, double-stranded regions of flap DNA.
About this Structure
1A77 is a Single protein structure of sequence from Methanocaldococcus jannaschii with MG as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of flap endonuclease-1 from Methanococcus jannaschii., Hwang KY, Baek K, Kim HY, Cho Y, Nat Struct Biol. 1998 Aug;5(8):707-13. PMID:9699635
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