2vg8

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[[Image:2vg8.jpg|left|200px]]
[[Image:2vg8.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2vg8 |SIZE=350|CAPTION= <scene name='initialview01'>2vg8</scene>, resolution 1.75&Aring;
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The line below this paragraph, containing "STRUCTURE_2vg8", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Udp+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Trs+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Edo+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Edo+Binding+Site+For+Chain+A'>AC4</scene>, <scene name='pdbsite=AC5:Edo+Binding+Site+For+Chain+A'>AC5</scene>, <scene name='pdbsite=AC6:Edo+Binding+Site+For+Chain+A'>AC6</scene>, <scene name='pdbsite=AC7:Edo+Binding+Site+For+Chain+A'>AC7</scene>, <scene name='pdbsite=AC8:Edo+Binding+Site+For+Chain+A'>AC8</scene>, <scene name='pdbsite=AC9:Edo+Binding+Site+For+Chain+A'>AC9</scene>, <scene name='pdbsite=BC1:Edo+Binding+Site+For+Chain+A'>BC1</scene> and <scene name='pdbsite=BC2:Edo+Binding+Site+For+Chain+A'>BC2</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UDP:URIDINE-5&#39;-DIPHOSPHATE'>UDP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydroquinone_glucosyltransferase Hydroquinone glucosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.218 2.4.1.218] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2vg8| PDB=2vg8 | SCENE= }}
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|RELATEDENTRY=[[2vce|2VCE]], [[2vch|2VCH]], [[2vcu|2VCU]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vg8 OCA], [http://www.ebi.ac.uk/pdbsum/2vg8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2vg8 RCSB]</span>
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}}
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'''CHARACTERIZATION AND ENGINEERING OF THE BIFUNCTIONAL N- AND O-GLUCOSYLTRANSFERASE INVOLVED IN XENOBIOTIC METABOLISM IN PLANTS'''
'''CHARACTERIZATION AND ENGINEERING OF THE BIFUNCTIONAL N- AND O-GLUCOSYLTRANSFERASE INVOLVED IN XENOBIOTIC METABOLISM IN PLANTS'''
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==About this Structure==
==About this Structure==
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2VG8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure supersedes the now removed PDB entry 2VCU. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VG8 OCA].
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2VG8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2vcu 2vcu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VG8 OCA].
==Reference==
==Reference==
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[[Category: Offen, W A.]]
[[Category: Offen, W A.]]
[[Category: Revett, T J.]]
[[Category: Revett, T J.]]
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[[Category: glycosyltransferase]]
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[[Category: Glycosyltransferase]]
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[[Category: n-glucosyltransferase]]
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[[Category: N-glucosyltransferase]]
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[[Category: n-glycosylation]]
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[[Category: N-glycosylation]]
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[[Category: o-glucosyltransferase]]
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[[Category: O-glucosyltransferase]]
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[[Category: plant glycosylation]]
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[[Category: Plant glycosylation]]
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[[Category: s-glucosyltransferase]]
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[[Category: S-glucosyltransferase]]
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[[Category: transferase]]
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[[Category: Transferase]]
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[[Category: udp-glucose- dependent]]
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[[Category: Udp-glucose- dependent]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:44:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:11:48 2008''
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Revision as of 15:44, 4 May 2008

Image:2vg8.jpg

Template:STRUCTURE 2vg8

CHARACTERIZATION AND ENGINEERING OF THE BIFUNCTIONAL N- AND O-GLUCOSYLTRANSFERASE INVOLVED IN XENOBIOTIC METABOLISM IN PLANTS


Overview

The glucosylation of pollutant and pesticide metabolites in plants controls their bioactivity and the formation of subsequent chemical residues. The model plant Arabidopsis thaliana contains >100 glycosyltransferases (GTs) dedicated to small-molecule conjugation and, whereas 44 of these enzymes catalyze the O-glucosylation of chlorinated phenols, only one, UGT72B1, shows appreciable N-glucosylating activity toward chloroanilines. UGT72B1 is a bifunctional O-glucosyltransferase (OGT) and N-glucosyltransferase (NGT). To investigate this unique dual activity, the structure of the protein was solved, at resolutions up to 1.45 A, in various forms including the Michaelis complex with intact donor analog and trichlorophenol acceptor. The catalytic mechanism and basis for O/N specificity was probed by mutagenesis and domain shuffling with an orthologous enzyme from Brassica napus (BnUGT), which possesses only OGT activity. Mutation of BnUGT at just two positions (D312N and F315Y) installed high levels of NGT activity. Molecular modeling revealed the connectivity of these residues to H19 on UGT72B1, with its mutagenesis exclusively defining NGT activity in the Arabidopsis enzyme. These results shed light on the conjugation of nonnatural substrates by plant GTs, highlighting the catalytic plasticity of this enzyme class and the ability to engineer unusual and desirable transfer to nitrogen-based acceptors.

About this Structure

2VG8 is a Single protein structure of sequence from Arabidopsis thaliana. This structure supersedes the now removed PDB entry 2vcu. Full crystallographic information is available from OCA.

Reference

Characterization and engineering of the bifunctional N- and O-glucosyltransferase involved in xenobiotic metabolism in plants., Brazier-Hicks M, Offen WA, Gershater MC, Revett TJ, Lim EK, Bowles DJ, Davies GJ, Edwards R, Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20238-43. Epub 2007 Dec 12. PMID:18077347 Page seeded by OCA on Sun May 4 18:44:34 2008

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