1a9v
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1a9v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a9v" /> '''TERTIARY STRUCTURE OF THE MAJOR HOUSE DUST M...)
Next diff →
Revision as of 08:35, 20 November 2007
|
TERTIARY STRUCTURE OF THE MAJOR HOUSE DUST MITE ALLERGEN DER P 2, NMR, 10 STRUCTURES
Overview
Sensitization to indoor allergens, especially those of the house dust, mite, is strongly correlated with the development of asthma. We report the, tertiary structure of the major house dust mite allergen, Der p 2, determined by NMR methods. The structure of Der p 2 is a beta-barrel and, is composed of two three-stranded antiparallel beta-pleated sheets. This, arrangement of beta-strands is similar to the immunoglobulin fold with, respect to the orientation of the two sheets and the interactions of the, strands. However, the three-dimensional structure of Der p 2 aligns, equivalently with a number of proteins from different families within the, immunoglobulin superfamily. The structural homology with the highest, significance score from analysis by DALI is to Der f 2. Although Der p 2, and Der f 2 are 87% identical in amino acid sequence, they align in three, dimensions rather poorly (4.85 A RMSD; Z-score, 8.58). This unexpected, finding is likely due to the different solution conditions used during, structure determination by NMR for both proteins. While the structural, comparisons did not elucidate a clear homologue for the function of Der p, 2 in mites, we report that Der p 2 is sequentially homologous to esr16., This is a protein from moths that is expressed coincident with molting., Thus, this homology has important ramifications for the study of mite, allergy. The structure of Der p 2 provides a useful tool in the design of, recombinant immunotherapeutics for the group 2 allergens.
About this Structure
1A9V is a Single protein structure of sequence from Dermatophagoides pteronyssinus. Full crystallographic information is available from OCA.
Reference
Tertiary structure of the major house dust mite allergen Der p 2: sequential and structural homologies., Mueller GA, Benjamin DC, Rule GS, Biochemistry. 1998 Sep 15;37(37):12707-14. PMID:9737847
Page seeded by OCA on Tue Nov 20 10:42:30 2007
