2vh4
From Proteopedia
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'''STRUCTURE OF A LOOP C-SHEET SERPIN POLYMER''' | '''STRUCTURE OF A LOOP C-SHEET SERPIN POLYMER''' | ||
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[[Category: Whisstock, J C.]] | [[Category: Whisstock, J C.]] | ||
[[Category: Zhang, Q.]] | [[Category: Zhang, Q.]] | ||
| - | [[Category: | + | [[Category: Hydrolase inhibitor]] |
| - | [[Category: | + | [[Category: Serpin,c sheet polymer,latent conformation]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:48:05 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:48, 4 May 2008
STRUCTURE OF A LOOP C-SHEET SERPIN POLYMER
Overview
In this study, we report the X-ray crystal structure of an N-terminally truncated variant of the bacterial serpin, tengpin (tengpinDelta42). Our data reveal that tengpinDelta42 adopts a variation of the latent conformation in which the reactive center loop is hyperinserted into the A beta-sheet and removed from the vicinity of the C-sheet. This conformational change leaves the C beta-sheet completely exposed and permits antiparallel edge-strand interactions between the exposed portion of the reactive center loop of one molecule and strand s2C of the C beta-sheet of the neighboring molecule in the crystal lattice. Our structural data thus reveal that tengpinDelta42 forms a loop C-sheet polymer in the crystal lattice. In vivo serpins have a propensity to misfold and form long-chain polymers, a process that underlies serpinopathies such as emphysema, thrombosis and dementia. Native serpins are thought to polymerize via a loop A-sheet mechanism. However, studies on plasminogen activator inhibitor 1 and the S49P variant of human neuroserpin reveal that the latent form of these molecules can also polymerize. Polymerization of latent neuroserpin may be important for the development of familial encephalopathy with neuroserpin inclusion bodies. Our structural data provide a possible mechanism for polymerization by latent serpins.
About this Structure
2VH4 is a Single protein structure of sequence from Thermoanaerobacter tengcongensis. Full crystallographic information is available from OCA.
Reference
A structural basis for loop C-sheet polymerization in serpins., Zhang Q, Law RH, Bottomley SP, Whisstock JC, Buckle AM, J Mol Biol. 2008 Mar 7;376(5):1348-59. Epub 2008 Jan 3. PMID:18234218 Page seeded by OCA on Sun May 4 18:48:05 2008
