2vkx

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[[Image:2vkx.jpg|left|200px]]
[[Image:2vkx.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2vkx |SIZE=350|CAPTION= <scene name='initialview01'>2vkx</scene>, resolution 2.7&Aring;
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The line below this paragraph, containing "STRUCTURE_2vkx", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Chain+C'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Chain+C'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Chain+F'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Chain+F'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Chain+A'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Chain+F'>AC7</scene>, <scene name='pdbsite=AC8:So4+Binding+Site+For+Chain+E'>AC8</scene>, <scene name='pdbsite=AC9:So4+Binding+Site+For+Chain+D'>AC9</scene>, <scene name='pdbsite=BC1:So4+Binding+Site+For+Chain+F'>BC1</scene> and <scene name='pdbsite=BC2:So4+Binding+Site+For+Chain+F'>BC2</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2vkx| PDB=2vkx | SCENE= }}
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|RELATEDENTRY=[[2vkw|2VKW]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vkx OCA], [http://www.ebi.ac.uk/pdbsum/2vkx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2vkx RCSB]</span>
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}}
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'''HUMAN NCAM, FN3 DOMAINS 1 AND 2, M610R MUTANT'''
'''HUMAN NCAM, FN3 DOMAINS 1 AND 2, M610R MUTANT'''
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[[Category: Hohenester, E.]]
[[Category: Hohenester, E.]]
[[Category: Saffell, J L.]]
[[Category: Saffell, J L.]]
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[[Category: adhesion receptor]]
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[[Category: Adhesion receptor]]
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[[Category: cell adhesion]]
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[[Category: Cell adhesion]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:58:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:13:23 2008''
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Revision as of 15:58, 4 May 2008

Image:2vkx.jpg

Template:STRUCTURE 2vkx

HUMAN NCAM, FN3 DOMAINS 1 AND 2, M610R MUTANT


Overview

Activation of the fibroblast growth factor receptor (FGFR) by neural cell adhesion molecule (NCAM) is essential for NCAM-mediated neurite outgrowth. Previous peptide studies have identified two regions in the fibronectin type 3 (FN3)-like domains of NCAM as being important for these activities. Here we report the crystal structure of the NCAM FN3 domain tandem, which reveals an acutely bent domain arrangement. Mutation of a non-conserved surface residue (M610R) led to a second crystal form showing a substantially different conformation. Thus, the FN3 domain linker is highly flexible, suggesting that it corresponds to the hinge seen in electron micrographs of NCAM. The two putative FGFR1-binding segments, one in each NCAM FN3 domain, are situated close to the domain interface. They form a contiguous patch in the more severely bent conformation but become separated upon straightening of the FN3 tandem, suggesting that conformational changes within NCAM may modulate FGFR1 activation. Surface plasmon resonance experiments demonstrated only a very weak interaction between the NCAM FN3 tandem and soluble FGFR1 proteins expressed in mammalian cells (dissociation constant >100 muM). Thus, the NCAM-FGFR1 interaction at the cell surface is likely to depend upon avidity effects due to receptor clustering.

About this Structure

2VKX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of the Tandem Fibronectin Type 3 Domains of Neural Cell Adhesion Molecule., Carafoli F, Saffell JL, Hohenester E, J Mol Biol. 2008 Jan 17;. PMID:18261743 Page seeded by OCA on Sun May 4 18:58:46 2008

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